PXD032256

PXD032256 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Priestia megaterium response to mupirocin treatment
Description	Isoleucyl-tRNA synthetase (IleRS) is an essential enzyme that covalently couples isoleucine to the corresponding tRNA. Bacterial IleRSs group in two clades, ileS1 and ileS2, the latter bringing resistance to the natural antibiotic mupirocin. Generally, bacteria rely on either ileS1 or ileS2 as a standalone housekeeping gene. However, we have found an exception in Bacillus species: in family Bacillaceae ileS1 appears mandatory, but some of the Bacilli posses additional, ileS2 gene. Taking Priestia (Bacillus) megaterium as a model organism, we showed that PmIleRS1 is constitutively expressed, while PmIleRS2 is stress-induced. As part of our investigation of ileS1/ileS2 function, we explored the expression of ileS1 and ileS2 under control conditions and upon mupirocin treatment.
HostingRepository	jPOST
AnnounceDate	2022-08-28
AnnouncementXML	Submission_2023-02-12_23:28:30.356.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032256
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marko Mocibob
SpeciesList	scientific name: Priestia megaterium NBRC 15308 = ATCC 14581; NCBI TaxID: 1348623;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-14 02:50:23	ID requested
1	2022-08-27 22:39:52	announced
2	2022-09-18 02:58:40	announced	2022-09-18: Updated FTP location.
⏵ 3	2023-02-12 23:28:31	announced	2023-02-13: Updated PubMed.

Publication List

Zanki V, Bozic B, Mocibob M, Ban N, Gruic-Sovulj I, A pair of isoleucyl-tRNA synthetases in Bacilli fulfills complementary roles to keep fast translation and provide antibiotic resistance. Protein Sci, 31(9):e4418(2022) [pubmed]

Zanki V, Bozic B, Mocibob M, Ban N, Gruic-Sovulj I, A pair of isoleucyl-tRNA synthetases in Bacilli fulfills complementary roles to keep fast translation and provide antibiotic resistance. Protein Sci, 31(9):e4418(2022) [pubmed]

Keyword List

submitter keyword: Priestia megaterium, Bacillus megaterium, mupirocin, isoleucyl-tRNA synthetase

submitter keyword: Priestia megaterium, Bacillus megaterium, mupirocin, isoleucyl-tRNA synthetase

Contact List

Ita Gruic-Sovulj
lab head
Marko Mocibob
contact affiliation	University of Zagreb, Faculty of Science
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001530/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001530/