PXD032255

PXD032255 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Subclassification of Extracellular Vesicles Based on Surface Glycan Structures by Spongy-like Separation Media
Description	This report develops a novel lectin-based affinity chromatography (LAC) method for extracellular vesicles (EVs) and demonstrates the possibility of subclassification based on their surface glycan.　EVs are lipid bilayer vesicles enclosing various biomolecules and represent their promising potential as a sensitive biomarker for detecting and monitoring various diseases. However, EVs have heterogeneous molecular compositions, even from identical donor cells by the same purification methods, which is a significant obstacle to elucidating objective biological functions. Therefore, subclassification based on their molecular compositions has been an urgent subject for addressing their heterogeneity. Here, as a novel LAC platform for EVs, we utilized a spongy-like monolithic polymer (named spongy monolith, SPM), which consists of poly(ethylene-co-glycidyl methacrylate) with continuous micropores that allowed efficient in-situ protein reaction with the epoxy groups. Two distinct lectins with different specificity, Sambucus sieboldiana agglutinin and concanavalin A, were effectively immobilized on SPM with remaining binding activity. Moreover, the large flow-through pores (>10 μm) of the SPM allowed high recovery rates of liposomal nanoparticles as a model of EVs. Finally, we employed lectin-immobilized SPMs for the subclassification of EVs based on their surface glycan structures and demonstrated their different subpopulations by proteome profiling.
HostingRepository	jPOST
AnnounceDate	2023-03-14
AnnouncementXML	Submission_2023-03-13_08:00:07.589.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eisuke Kanao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-14 00:58:48	ID requested
⏵ 1	2023-03-13 08:00:07	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: extracellular vesicle, glycan structure, heterogeneity

submitter keyword: extracellular vesicle, glycan structure, heterogeneity

Contact List

Yasushi Ishihama
lab head
Eisuke Kanao
contact affiliation	Graduate School of Pharmaceutical Sciences, Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001529/