PXD032241

PXD032241 is an original dataset announced via ProteomeXchange.

Title	Label-free shot-proteomics of S. cerevisiae producing starch-like glucans
Description	The synthesis of starch-like glucans can be reconstituted in S. cerevisiae using the genes from Arabidopsis thaliana: heterologous expression of SS1 to SS4, BE2, BE3, ISA1, and ISA2, together with a bacterial ADPglucose pyrophosphorylase gene for substrate provision in yeast strain 29, yielded insoluble granules with a semi-crystalline structure similar to that of plant amylopectin (Pfister et al., 2016). Here we conducted a label-free proteomics experiment to compare yeast strains with and without glucan production. This experiment was performed on strains 29, 48A, 362.1, 363.1 and WT (n = 4 replicates each, arising from independent replicate cultures). Strains including genotypes are described in the associated publication. Reference: Pfister, B., Sánchez-Ferrer, A., Diaz, A., Lu, K., Otto, C., Holler, M., Shaik, F.R., Meier, F., Mezzenga, R., and Zeeman, S.C. (2016). Recreating the synthesis of starch granules in yeast. Elife 5: 1–29.
HostingRepository	PRIDE
AnnounceDate	2022-09-05
AnnouncementXML	Submission_2022-09-05_02:09:40.584.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Pfister
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 11:26:21	ID requested
⏵ 1	2022-09-05 02:09:40	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, ProgenesisQI, yeast

Samuel Christian Zeeman
contact affiliation	Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Switzerland
contact email	szeeman@ethz.ch
lab head
Barbara Pfister
contact affiliation	ETH Zurich
contact email	bpfister@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032241
     1. Label: PRIDE project
     2. Name: Label-free shot-proteomics of S. cerevisiae producing starch-like glucans