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PXD032240

PXD032240 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnbiased in vivo exploration of nuclear bodies-enhanced sumoylation reveals that PML orchestrates embryonic stem cell fate (II)
DescriptionMembrane-less organelles are condensates formed by phase separation whose functions often remain enigmatic. Upon oxidative stress, PML scaffolds Nuclear Bodies (NBs) to regulate senescence or metabolic adaptation, but their role in pluripotency remains elusive. Here we establish that PML is required for basal SUMO2/3 conjugation in mESCs and oxidative stress-driven sumoylation in mESCs or in vivo. PML NBs create an oxidationprotective environment for UBC9-driven SUMO2/3 conjugation of PML partners, often followed by their poly-ubiquitination and degradation. Differential in vivo proteomics identified several members of the KAP1 complex as PML NB-dependent SUMO2-targets. The latter drives functional activation of this key epigenetic repressor. Accordingly, Pml-/- mESCs reexpress transposable elements and display features of totipotent-like cells, a process further enforced by PML-controlled SUMO2-conjugation of DPPA2. Finally, PML is required for adaptive stress responses in mESCs. Collectively, PML orchestrates mESC fate through SUMO2-conjugation of key transcriptional or epigenetic regulators, raising new mechanistic hypotheses about PML roles in normal or cancer stem cells.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:24:33.867.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomán González-Prieto
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-11 11:16:34ID requested
12023-03-06 01:46:32announced
22023-11-14 07:24:35announced2023-11-14: Updated project metadata.
Publication List
Tessier S, Ferhi O, Geoffroy MC, Gonz, á, lez-Prieto R, Canat A, Quentin S, Pla M, Niwa-Kawakita M, Bercier P, R, é, rolle D, Tirard M, Therizols P, Fabre E, Vertegaal ACO, de Th, é H, Lallemand-Breitenbach V, Author Correction: Exploration of nuclear body-enhanced sumoylation reveals that PML represses 2-cell features of embryonic stem cells. Nat Commun, 14(1):124(2023) [pubmed]
Keyword List
submitter keyword: Proteomics, SUMO,PML
Contact List
Alfred C.O. Vertegaal
contact affiliationCell and Chemical Biology Leiden University Medical Center Leiden The Netherlands
contact emailvertegaal@lumc.nl
lab head
Román González-Prieto
contact affiliationAndalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact emailroman.gonzalez@cabimer.es
dataset submitter
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Dataset FTP location
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