PXD032223

PXD032223 is an original dataset announced via ProteomeXchange.

Title	Systems-level nutrient stress analysis of soil grown Brassica napus shoots and roots by quantitative proteomics
Description	Appropriate soil nutrient management is critical for modern Canola (Brassica napus L.) varieties and hybrids to meet their yield potentials. Canola fields are typically supplemented by the application of nitrogen, phosphorus, sulphur, and to a lesser extent, potassium fertilizers to maximize yield, while deficiency in these key macronutrients can result in severe growth phenotypes and significant yield losses. To date, our understanding of canola nutrient deficiency responses is disparate, encompassing a large number of varieties using a variety of technologies and assessment criteria, with seminal understanding of the overlaps between nutrient deficiencies responses, having not yet been defined, placing limitations on our ability to increase the nutrient efficiency of this critical oil seed. To address this, we performed a comparative quantitative proteomics analysis of both shoot and root tissue harvested from soil-grown Canola plants experiencing either nitrogen, phosphorus, potassium, or sulphur deficiency. Our results show intriguing similarities in plant responses to deficiency in multiple nutrients. We also find very distinct proteome-level changes between shoot and root tissue of plants experiencing nutrient stress, suggesting the presence of highly organ-specific responses to nutrient deficiency. Our results pave the way for a more comprehensive understanding of the shared and distinct response mechanisms of plants to multiple essential nutrients.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:04:50.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 10:35:54	ID requested
⏵ 1	2025-05-06 09:04:51	announced

Grubb LE, Scandola S, Mehta D, Khodabocus I, Uhrig RG, Quantitative Proteomic Analysis of Brassica Napus Reveals Intersections Between Nutrient Deficiency Responses. Plant Cell Environ, 48(2):1409-1428(2025) [pubmed]

10.1111/pce.15216;

submitter keyword: BoxCarDIA, nutrient stress, roots, shoots,Brassica napus, quantitative proteomics

Richard Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD032223

PRIDE project URI

• PRIDE
  1. PXD032223
     1. Label: PRIDE project
     2. Name: Systems-level nutrient stress analysis of soil grown Brassica napus shoots and roots by quantitative proteomics