PXD032219

PXD032219 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics Landscape of Asymptomatic and Symptomatic Human Alzheimer's Disease Brain
Description	Alterations of protein abundance and post-translational modifications in patients with Alzheimer’s disease (AD), such as glycosylation, phosphorylation, and ubiquitination, and their roles in disease progression and treatment outcome are areas of intense study. Little is known, however, about the overall N-glycosylation of proteins in human brains, and those from Alzheimer’s patients, particularly in regard to large-scale intact N-linked glycoproteomics analysis. To elucidate the glycoproteome landscape, we developed an approach based on multi-lectin affinity enrichment, hydrophilic interaction chromatography (HILIC), and LC-MS-based glycoproteomics analysis. We analyzed 10 normal, 10 asymptomatic, and 10 symptomatic AD brains, in which we detected >300 glycoproteins and >1,900 glycoforms across the samples. The majority of glycoproteins have N-glycans that are high-mannosidic and complex chains that are fucosylated and bisected. The Man5 N-glycan was found to occur most frequently at >20% of the total glycoforms. Unlike the glycoproteomes of other tissues, sialylation is a minor feature of the brain glycoproteome, occurring at <9% of N-glycans . We observed changes in the number of antennae, frequency of fucosylation, bisection, and other monosaccharides at individual glycosylation sites among normal, asymptomatic, and symptomatic AD samples. Further analysis revealed glycosylation differences in subcellular compartments. We did not observe a statistical difference between male and female patients. These results represent the first glycoproteomics landscape of brains from multiple AD individuals, which will facilitate a deeper understanding of AD and possible disease treatment options.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:20.468.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suttipong Suttapitugsakul
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 10:03:37	ID requested
1	2022-10-31 08:29:13	announced
⏵ 2	2023-11-14 08:30:21	announced	2023-11-14: Updated project metadata.

Suttapitugsakul S, Stavenhagen K, Donskaya S, Bennett DA, Mealer RG, Seyfried NT, Cummings RD, Glycoproteomics Landscape of Asymptomatic and Symptomatic Human Alzheimer's Disease Brain. Mol Cell Proteomics, 21(12):100433(2022) [pubmed]

submitter keyword: glycoproteins, mass spectrometry, glycoproteomics,Alzheimer's

Richard D. Cummings
contact affiliation	Beth Israel Deaconess Medical Center and Harvard Medical School
contact email	rcummin1@bidmc.harvard.edu
lab head
Suttipong Suttapitugsakul
contact affiliation	Beth Israel Deaconess Medical Center and Harvard Medical School
contact email	ssuttapi@bidmc.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032219

PRIDE project URI

• PRIDE
  1. PXD032219
     1. Label: PRIDE project
     2. Name: Glycoproteomics Landscape of Asymptomatic and Symptomatic Human Alzheimer's Disease Brain