PXD032203

PXD032203 is an original dataset announced via ProteomeXchange.

Title	Detection of Cell-Cell Interactions via Photocatalytic Cell Tagging
Description	The growing appreciation of immune cell-cell interactions within disease environments has led to significant efforts to develop highly effective protein- and cell-based immunotherapies. However, characterizing these complex cell-cell interfaces in high resolution remains challenging. Thus, technologies that can leverage therapeutic-based modalities to profile intercellular environments offer the opportunity to study cell-cell interactions with molecular-level insight. To address this, we introduce Photocatalytic Cell Tagging (PhoTag), a platform for profiling cell-cell interactions utilizing a single domain antibody (VHH) conjugated to a photoactivatable flavin-based cofactor. Upon irradiation with visible light, the tethered flavin photocatalyst generates phenoxy radical tags for targeted labeling within cell-cell contact regions. Using anti-PD-1 or anti-PD-L1 VHH flavin conjugates, we demonstrate that PhoTag achieves highly selective synaptic labeling in antigen presenting cell-T cell co-culture systems. By combining the high resolution transcellular biotinylation capability of PhoTag with multi-omics single cell sequencing, we interrogated transient interactions between peripheral blood mononuclear cell (PBMC) populations and Raji PD-L1 B cells and discovered that specific T cell subtypes can transiently interact more efficiently than others. We envision that the spatiotemporal and modular nature of PhoTag will enable its broad utilization for detailed profiling of intercellular interactions across different biological systems.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:20.558.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032203
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rob Oslund
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 09:23:28	ID requested
1	2022-06-03 05:21:10	announced
⏵ 2	2023-11-14 08:55:21	announced	2023-11-14: Updated project metadata.

10.6019/PXD032203;

submitter keyword: Flavin-based photocatalysis, phenoxy radical generation, tyrosine labeling

Rob Oslund
contact affiliation	PhD
contact email	rob.oslund@gmail.com
lab head
Rob Oslund
contact affiliation	Merck Exploratory Science Center
contact email	rob.oslund@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032203

PRIDE project URI

• PRIDE
  1. PXD032203
     1. Label: PRIDE project
     2. Name: Detection of Cell-Cell Interactions via Photocatalytic Cell Tagging