PXD032202

PXD032202 is an original dataset announced via ProteomeXchange.

Title	Global and comparative proteome signatures in the lens capsule, trabecular meshwork, and iris of patients with pseudoexfoliation glaucoma
Description	Pseudoexfoliation (PXF) is characterized by the accumulation of exfoliative material in the eye and high rates of blindness, if left untreated. Pseudoexfoliation glaucoma (PXG) is generally diagnosed too late due to its asymptomatic nature, necessitating the development of new effective screening tools for the early diagnosis of the disease. Thus, the increasing prevalence of this disease due to an aging population has necessitated the identification of suitable biomarkers for the early detection of the disease or detection of the onset of glaucoma in eyes with PXF. We applied a proteomics strategy based on a high-throughput screening method for the determination of proteins involving PXF and PXG pathogenesis. Lens capsule (LC) samples were obtained from surgical trabeculectomy of 146 adults with PXF, PXG, and controls to characterize the proteome profile. Peptides from LC were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The protein of interest and cytokines/chemokines profiles were verified using immunohistochemistry and bio-plex kit assay, respectively. We have also analyzed the proteomic profile of trabecular meshwork (TM) and iris samples with PXG compared to control. There were 1433 proteins identified in human LC, of which 27 proteins were overexpressed and 8 proteins were under-expressed in PXG compared with PXF. Overexpressed proteins such as fibromodulin, decorin, lysyl oxidase homolog 1, Collagen alpha-1(I) chain, and Collagen alpha-3(VI) chain were the major components of the extracellular matrix (ECM) proteins involved in cell-matrix interactions or ECM proteoglycans, assembly and crosslinking of collagen fibrils. The ECM composition and homeostasis are altered in glaucoma; thus, quantitative proteomics is a method to discover molecular markers in the eye. Monitoring of these events can help evaluate disease progression in future studies.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_08:39:47.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prity Sahay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 09:15:53	ID requested
⏵ 1	2022-05-20 08:39:48	announced

Sahay P, Chakraborty M, Rao A, Global and Comparative Proteome Signatures in the Lens Capsule, Trabecular Meshwork, and Iris of Patients With Pseudoexfoliation Glaucoma. Front Mol Biosci, 9():877250(2022) [pubmed]

submitter keyword: pseudoexfoliation (PXF), pseudoexfoliation glaucoma (PXG), lens capsule, lysyl oxidase, trabecular meshwork, iris, mass spectroscopy, extracellular matrix

Dr. Aparna Rao
contact affiliation	L V Prasad Eye Institute, Mithu Tulsi Chanrai Campus Patia, Bhubaneswar
contact email	vinodini10375@yahoo.com
lab head
Prity Sahay
contact affiliation	L.V. Prasad Eye Institute
contact email	prity_sahay08@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032202
     1. Label: PRIDE project
     2. Name: Global and comparative proteome signatures in the lens capsule, trabecular meshwork, and iris of patients with pseudoexfoliation glaucoma