PXD032165

PXD032165 is an original dataset announced via ProteomeXchange.

Title	NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization
Description	Chromatin insulators are DNA-protein complexes that can prevent the spread of repressive chromatin and block communication between enhancers and promoters to regulate gene expression. In Drosophila, the gypsy chromatin insulator complex consists of three core proteins: CP190, Su(Hw), and Mod(mdg4)67.2. These factors concentrate at nuclear foci termed insulator bodies, and their normal localization is correlated with proper insulator function. Here, we identified NURF301/E(bx), a nucleosome remodeling factor, as a novel regulator of gypsy insulator body localization through a high-throughput RNAi imaging screen. NURF301 promotes gypsy-dependent insulator barrier activity and physically interacts with gypsy insulator proteins. Using ChIP-seq, we found that NURF301 co-localizes with insulator proteins genome-wide, and NURF301 promotes chromatin association of Su(Hw) and CP190 at gypsy insulator binding sites. These effects correlate with NURF301-dependent nucleosome repositioning. At the same time, CP190 and Su(Hw) are also required for recruitment of NURF301 to chromatin. Finally, Oligopaint FISH combined with immunofluorescence revealed that NURF301 promotes 3D contact between insulator bodies and gypsy binding site DNA, and NURF301 is required for proper nuclear positioning of gypsy binding sites. Our data provide new insights into how a nucleosome remodeling factor and insulator proteins cooperatively contribute to nuclear organization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:09:35.009.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032165
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	shue chen
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-methionine sulfoxide; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:34:41	ID requested
1	2022-10-14 16:01:28	announced
⏵ 2	2023-11-14 08:09:38	announced	2023-11-14: Updated project metadata.

Chen S, Rosin LF, Pegoraro G, Moshkovich N, Murphy PJ, Yu G, Lei EP, NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization. Nucleic Acids Res, 50(14):7906-7924(2022) [pubmed]

10.6019/PXD032165;

submitter keyword: mass spec, NURF301,drosophila, gypsy insulator

Elissa Lei
contact affiliation	Laboratory of Biochemistry and Genetics, NIDDK, NIH, USA
contact email	elissalei@niddk.nih.gov
lab head
shue chen
contact affiliation	NIH
contact email	chenshue001@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032165
     1. Label: PRIDE project
     2. Name: NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization