Updated project metadata. Rice (Oryza staiva) is staple food for the human consumption and model plants for genetic and molecular studies. Only few studies have been conducted on ultraviolet-B (UV-B) stress on rice. UV-B stress effects different morphological and physiological changes in plants. However, underlying mechanisms governing these integrated responses are unknown. In this study, we conducted proteomic response of rice leaves to UV-B stress using two-dimensional gel electrophoresis and identified the selected proteins by mass spectrometry analysis. To identify changes in protein accumulation in response to UV-B stress, two cultivars, i.e., IR6 and REX were irradiated by artificial UV-B radiation. Four 4 levels of daily biologically effective UV-B radiation intensities, 0 (control), 5, 10, and 15 kJ m-2 d-1 were imposed. We conducted this experiment in Sunlit Soil-Plant-Atmosphere-Research (SPAR) chambers to mimic the natural environment.