PXD032160

PXD032160 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial dynamics in ovarian granulosa cells during follicular growth and in human KGN cells
Description	In the ovary, proliferation and differentiation of granulosa cells (GCs) drive the growth of follicles. This is, in part, dependent on gonadotropins. Our immunohistochemical studies provided hints of mitochondrial biogenesis and intracellular redistribution in GCs of growing follicles. A cellular model, human KGN cells (granulosa cell tumor cells, derived from growing follicles) was used to study aspects of mitochondrial dynamics. To elevate cAMP and thereby mimic gonadotropin actions, forskolin (FSK) was used, which increased KGN cell size and mitochondrial DNA within 24 h. MitoTracker experiments and ultrastructural 3D reconstruction revealed that FSK treatment induced the formation of elaborate mitochondrial networks. H89, a protein kinase A (PKA) inhibitor, reduced network formation. A proteomic analysis indicated that FSK among others elevated levels of regulators of the cytoskeleton and the steroidogenic enzyme CYP11A1, located in mitochondria, was more than 3-fold increased, implying that cAMP/PKA-associated structural changes go in parallel with the acquisition of steroidogenic competence of mitochondria in KGN cells. In summary, in situ observations showed increases in mitochondria and suggested intracellular trafficking in GCs during follicular growth and indicated that they may partially be under the control of gonadotropins/cAMP. In line with this, elevation of cAMP in KGN profoundly affected mitochondria dynamics in a PKA-dependent manner and implicated cytoskeletal changes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:59:34.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:26:44	ID requested
1	2022-10-14 15:12:58	announced
⏵ 2	2023-11-14 07:59:35	announced	2023-11-14: Updated project metadata.

Kaseder M, Schmid N, Eubler K, Goetz K, M, ü, ller-Taubenberger A, Dissen GA, Harner M, Wanner G, Imhof A, Forne I, Mayerhofer A, Evidence of a role for cAMP in mitochondrial regulation in ovarian granulosa cells. Mol Hum Reprod, 28(10):(2022) [pubmed]

submitter keyword: mitochondrial dynamics,Ovary, Forskolin, cAMP, KGN, granulosa cells

Prof. Dr. med. Artur Mayerhofer
contact affiliation	BMC, Zellbiologie, Anatomie III Großhaderener Strasse 9 D-82152 Planegg Germany
contact email	mayerhofer@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032160

PRIDE project URI

• PRIDE
  1. PXD032160
     1. Label: PRIDE project
     2. Name: Mitochondrial dynamics in ovarian granulosa cells during follicular growth and in human KGN cells