PXD032157

PXD032157 is an original dataset announced via ProteomeXchange.

Title	A male-specific steroid hormone controls female reproductive biology in the malaria mosquito
Description	Insects, unlike vertebrates, are generally believed to lack steroid hormones with functions predominantly associated with adult male biology. In the malaria mosquito Anopheles gambiae, the ecdysteroid 20-hydroxyecdysone (20E) appears to both control egg development in females and induce mating refractoriness and oviposition when sexually transferred by males. Here we show that these sex-specific functions are instead carried out by distinct steroids. We identify a male-specific oxidized form of 20E (3D20E) that upon sexual transfer switches off female mating receptivity, ensuring male paternity. Endogenous female 20E does not induce mating refractoriness, while it triggers oviposition in mated females when expression of a 20E-inhibiting kinase is repressed. 3D20E and 20E have different downstream targets, with 3D20E inducing expression of a tolerance factor that preserves female fitness during Plasmodium infection. The evolution of this male steroid has therefore not only shaped the mating biology of An. gambiae, but also impacted malaria transmission.
HostingRepository	PRIDE
AnnounceDate	2022-04-21
AnnouncementXML	Submission_2022-04-21_01:30:04.700.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gennifer Merrihew
SpeciesList	scientific name: Anopheles gambiae str. PEST; NCBI TaxID: 180454;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:23:47	ID requested
⏵ 1	2022-04-21 01:30:05	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, Mosquito, Ejaculome, QE-HF, malaria

Michael MacCoss
contact affiliation	MacCoss Lab University of Washington Department of Genome Sciences Seattle, WA USA
contact email	maccoss@uw.edu
lab head
Gennifer Merrihew
contact affiliation	Dept of Genome Sciences, University of Washington
contact email	genn@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032157
     1. Label: PRIDE project
     2. Name: A male-specific steroid hormone controls female reproductive biology in the malaria mosquito