PXD032156

PXD032156 is an original dataset announced via ProteomeXchange.

Title	Novel protein interaction network of human calcitonin receptor-like receptor revealed by label-free quantitative proteomics
Description	Background : The G protein-coupled receptor (GPCR) calcitonin receptor-like receptor (CLR) is implicated in cardiovascular and skin diseases, migraine and cancer. Beyond its agonists, receptor activity-modifying proteins and receptor component protein, interacting partners of this GPCR (‘CLR interactome’) are currently unknown. Herein, we defined CLR interactome in primary human dermal lymphatic endothelial cells (HDLEC). Immunoprecipitation (IP) of core- and terminally-glycosylated CLR and label-free quantitative mass spectrometry allowed the identification of 46 novel interaction partners from a total HDLEC proteome consisting of 4,902 proteins. Aims: The main aim of this study was to characterise the interactome of endogenous CLR along with the expression of this GPCR in the context of the proteomic profile of primary human dermal lymphatic endothelial cells cultured in vitro. Methods We used label-free quantitative proteomic analysis to analyse the CLR co-IP eluates and total protein lysates obtained from cultured in vitro primary human dermal lymphatic endothelial cells (from Promocell).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:57.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dimitrios Manolis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 02:21:24	ID requested
1	2024-08-09 05:21:19	announced
⏵ 2	2024-10-22 06:54:00	announced	2024-10-22: Updated project metadata.

10.1016/j.jbc.2024.107399;

Manolis D, Hasan S, Maraveyas A, O'Brien DP, Kessler BM, Kramer H, Nikitenko LL, Quantitative proteomics reveals CLR interactome in primary human cells. J Biol Chem, 300(6):107399(2024) [pubmed]

submitter keyword: skin.,Endothelial cell, human, lymphatic, proteome

Holger Kramer
contact affiliation	Proteomics and Metabolomics Facility, MRC London Institute of Medical Sciences, Hammersmith Hospital Campus, Du Cane Rd, London W12 0NN
contact email	h.kramer@lms.mrc.ac.uk
lab head
Dimitrios Manolis
contact affiliation	University of Hull
contact email	d.manolis-2017@hull.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD032156

PRIDE project URI

• PRIDE
  1. PXD032156
     1. Label: PRIDE project
     2. Name: Novel protein interaction network of human calcitonin receptor-like receptor revealed by label-free quantitative proteomics