PXD032131

PXD032131 is an original dataset announced via ProteomeXchange.

Title	Tankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death
Description	TNF is a key component of the innate immune response. Upon binding to its receptor, TNFR1, it promotes production of other cytokines via a membrane-bound complex 1, or induces cell death via a cytosolic complex 2. To understand how TNF-induced cell death is regulated we performed mass spectrometry of complex 2 and identified tankyrase-1 as a native component that, upon a death stimulus, mediates complex 2 poly-ADP-ribosylation (PARylation). PARylation promotes recruitment of the E3 ligase RNF146 resulting in proteasomal degradation of complex 2 thereby limiting cell death. Intriguingly, expression of the ADP-ribose binding/hydrolyzing SARS-CoV-2 macrodomain sensitizes cells to TNF-induced death via abolishing complex 2 PARylation. This suggests that disruption of ADP-ribosylation during an infection can prime a cell to retaliate with an inflammatory cell death.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_08:55:58.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deena Leslie Pedrioli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-09 00:35:48	ID requested
⏵ 1	2022-06-09 08:55:59	announced

Liu L, Sandow JJ, Leslie Pedrioli DM, Samson AL, Silke N, Kratina T, Ambrose RL, Doerflinger M, Hu Z, Morrish E, Chau D, Kueh AJ, Fitzibbon C, Pellegrini M, Pearson JS, Hottiger MO, Webb AI, Lalaoui N, Silke J, Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death. Sci Adv, 8(19):eabh2332(2022) [pubmed]

submitter keyword: ADP-ribosylation, Tankyrase, TNF-induced cell death

John Silke
contact affiliation	The Walter and Eliza Hall Institute of Medical Research Parkville VIC 3052, Australia Department of Medical Biology University of Melbourne Parkville VIC 3010, Australia
contact email	silke@wehi.edu.au
lab head
Deena Leslie Pedrioli
contact affiliation	University of Zürich
contact email	deena.lesliepedrioli@dmmd.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032131

PRIDE project URI

• PRIDE
  1. PXD032131
     1. Label: PRIDE project
     2. Name: Tankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death