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PXD032131

PXD032131 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death
DescriptionTNF is a key component of the innate immune response. Upon binding to its receptor, TNFR1, it promotes production of other cytokines via a membrane-bound complex 1, or induces cell death via a cytosolic complex 2. To understand how TNF-induced cell death is regulated we performed mass spectrometry of complex 2 and identified tankyrase-1 as a native component that, upon a death stimulus, mediates complex 2 poly-ADP-ribosylation (PARylation). PARylation promotes recruitment of the E3 ligase RNF146 resulting in proteasomal degradation of complex 2 thereby limiting cell death. Intriguingly, expression of the ADP-ribose binding/hydrolyzing SARS-CoV-2 macrodomain sensitizes cells to TNF-induced death via abolishing complex 2 PARylation. This suggests that disruption of ADP-ribosylation during an infection can prime a cell to retaliate with an inflammatory cell death.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_08:55:58.982.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDeena Leslie Pedrioli
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListadenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-09 00:35:48ID requested
12022-06-09 08:55:59announced
Publication List
Liu L, Sandow JJ, Leslie Pedrioli DM, Samson AL, Silke N, Kratina T, Ambrose RL, Doerflinger M, Hu Z, Morrish E, Chau D, Kueh AJ, Fitzibbon C, Pellegrini M, Pearson JS, Hottiger MO, Webb AI, Lalaoui N, Silke J, Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death. Sci Adv, 8(19):eabh2332(2022) [pubmed]
Keyword List
submitter keyword: ADP-ribosylation, Tankyrase, TNF-induced cell death
Contact List
John Silke
contact affiliationThe Walter and Eliza Hall Institute of Medical Research Parkville VIC 3052, Australia Department of Medical Biology University of Melbourne Parkville VIC 3010, Australia
contact emailsilke@wehi.edu.au
lab head
Deena Leslie Pedrioli
contact affiliationUniversity of Zürich
contact emaildeena.lesliepedrioli@dmmd.uzh.ch
dataset submitter
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Dataset FTP location
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