PXD032131 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death |
Description | TNF is a key component of the innate immune response. Upon binding to its receptor, TNFR1, it promotes production of other cytokines via a membrane-bound complex 1, or induces cell death via a cytosolic complex 2. To understand how TNF-induced cell death is regulated we performed mass spectrometry of complex 2 and identified tankyrase-1 as a native component that, upon a death stimulus, mediates complex 2 poly-ADP-ribosylation (PARylation). PARylation promotes recruitment of the E3 ligase RNF146 resulting in proteasomal degradation of complex 2 thereby limiting cell death. Intriguingly, expression of the ADP-ribose binding/hydrolyzing SARS-CoV-2 macrodomain sensitizes cells to TNF-induced death via abolishing complex 2 PARylation. This suggests that disruption of ADP-ribosylation during an infection can prime a cell to retaliate with an inflammatory cell death. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-09 |
AnnouncementXML | Submission_2022-06-09_08:55:58.982.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Deena Leslie Pedrioli |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | adenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-09 00:35:48 | ID requested | |
⏵ 1 | 2022-06-09 08:55:59 | announced | |
Publication List
Liu L, Sandow JJ, Leslie Pedrioli DM, Samson AL, Silke N, Kratina T, Ambrose RL, Doerflinger M, Hu Z, Morrish E, Chau D, Kueh AJ, Fitzibbon C, Pellegrini M, Pearson JS, Hottiger MO, Webb AI, Lalaoui N, Silke J, Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death. Sci Adv, 8(19):eabh2332(2022) [pubmed] |
Keyword List
submitter keyword: ADP-ribosylation, Tankyrase, TNF-induced cell death |
Contact List
John Silke |
contact affiliation | The Walter and Eliza Hall Institute of Medical Research Parkville VIC 3052, Australia Department of Medical Biology University of Melbourne Parkville VIC 3010, Australia |
contact email | silke@wehi.edu.au |
lab head | |
Deena Leslie Pedrioli |
contact affiliation | University of Zürich |
contact email | deena.lesliepedrioli@dmmd.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032131
- Label: PRIDE project
- Name: Tankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death