PXD032102

PXD032102 is an original dataset announced via ProteomeXchange.

Title	Calmodulin proximal interacting proteins revealed through BioID technology in the tachyzoite stage of Toxoplasma gondii
Description	Toxoplasma gondii is an apicomplexan parasite infecting human and animals, causing huge health concerns and economic losses. Calcium ion, a critical second messenger in cells, can regulate related vital activities, particularly in parasite invasion and escape processes. Calmodulin (CaM) is a short, highly conserved Ca2+ binding protein found in all eukaryotic cells, including apicomplexan parasites. After binding to Ca2+, CaM can be activated to interact with a variety of proteins (such as enzymes). Nevertheless, CaM-interacting proteins have not been identified in T. gondii. We report here the use of T. gondii strain RH△hxgprt expressing the proximity-labeling enzyme BirA* fused to CaM, in combination with LC-MS/MS to specifically identify CaM-interacting proteins. Our study revealed over three hundred of CaM’s proximal interacting proteins in T. gondii. These CaM partners were broadly dispersed throughout the parasite. The majority of their CRISPR fitness scores were below zero, indicating CaM's essential functions in parasites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:19.669.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032102
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yongle Song
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-07 03:51:28	ID requested
1	2022-09-25 03:13:51	announced
⏵ 2	2023-11-14 08:55:20	announced	2023-11-14: Updated project metadata.

10.6019/PXD032102;

submitter keyword: Toxoplasma gondii, Calmodulin, proximity-labeling, LC-MS/MS

Yanqin Zhou
contact affiliation	College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
contact email	yanqinzhou@mail.hzau.edu.cn
lab head
Yongle Song
contact affiliation	College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China
contact email	songyongle@webmail.hzau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD032102

PRIDE project URI

• PRIDE
  1. PXD032102
     1. Label: PRIDE project
     2. Name: Calmodulin proximal interacting proteins revealed through BioID technology in the tachyzoite stage of Toxoplasma gondii