PXD032085

PXD032085 is an original dataset announced via ProteomeXchange.

Title	Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling
Description	Viruses must effectively remodel host cellular pathways to replicate and evade immune defenses, and they must do so with limited genomic coding capacity. Targeting post-translational modification (PTM) pathways provides a mechanism by which viruses can broadly and rapidly transform a hostile host environment into a hospitable one. We used mass spectrometry-based proteomics to quantify changes in protein abundance and two PTM types – phosphorylation and ubiquitination – in response to HIV-1 infection with viruses harboring targeted deletions of a subset of HIV-1 genes. PTM analysis revealed a requirement for Aurora kinase activity in HIV-1 infection and identified substrates of a phosphatase that is degraded during infection. Finally, we demonstrated that the Cullin4A-DDB1-DCAF1 E3 ubiquitin ligase ubiquitinates histone H1 somatic isoforms and that the HIV-1 Vpr protein inhibits this process, leading to defects in DNA repair.
HostingRepository	PRIDE
AnnounceDate	2023-07-13
AnnouncementXML	Submission_2023-07-13_01:30:46.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JeffreyJohnson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 12:16:10	ID requested
1	2022-04-14 05:58:51	announced
⏵ 2	2023-07-13 01:30:47	announced	2023-07-13: Updated project metadata.

Johnson JR, Crosby DC, Hultquist JF, Kurland AP, Adhikary P, Li D, Marlett J, Swann J, H, ü, ttenhain R, Verschueren E, Johnson TL, Newton BW, Shales M, Simon VA, Beltrao P, Frankel AD, Marson A, Cox JS, Fregoso OI, Young JAT, Krogan NJ, Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling. Cell Rep, 39(2):110690(2022) [pubmed]

submitter keyword: HIV-1, proteomics, vif, b56, ubiquitination, vpr, pp2a, phosphorylation, histone h1, systems biology

JeffreyJohnson
contact affiliation	Department of Microbiology; Icahn School of Medicine at Mount Sinai; New York, NY, 10029; USA
contact email	Jeffrey.Johnson@mssm.edu
lab head
JeffreyJohnson
contact affiliation	Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032085

PRIDE project URI

• PRIDE
  1. PXD032085
     1. Label: PRIDE project
     2. Name: Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling