PXD032083 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of arginine phosphorylation in Mycolicibacterium smegmatis by shotgun proteomics |
Description | Tuberculosis is a leading cause of worldwide infectious mortality. The prevalence of multidrug-resistant Mycobacterium tuberculosis (Mtb) infections drives an urgent need to exploit new drug targets. One such target is the ATP-dependent protease ClpC1P1P2, which is strictly essential for viability. However, few proteolytic substrates of mycobacterial ClpC1P1P2 have been identified to date. Recent studies in Bacillus subtilis have shown that the orthologous ClpCP protease recognizes proteolytic substrates bearing post-translational arginine phosphorylation. While several lines of evidence suggest that ClpC1P1P2 is similarly capable of recognizing phosphoarginine-bearing proteins, the existence of phosphoarginine modifications in mycobacteria has remained in question. Here, we confirm the presence of post-translational phosphoarginine modifications in Mycolicibacterium smegmatis (Msm), a nonpathogenic surrogate of Mtb. Using a phosphopeptide enrichment workflow coupled with shotgun phosphoproteomics, we identify arginine phosphosites on several functionally diverse targets within the Msm proteome. Interestingly, phosphoarginine modifications are not upregulated by heat stress, suggesting divergent roles in mycobacteria and Bacillus. Our findings provide new evidence supporting the existence of phosphoarginine-mediated proteolysis by ClpC1P1P2 in mycobacteria and other actinobacterial species. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:55:21.884.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD032083 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Emmanuel Ogbonna |
SpeciesList | scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-05 12:15:13 | ID requested | |
1 | 2022-09-30 10:53:15 | announced | |
⏵ 2 | 2023-11-14 08:55:22 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: arginine phosphorylation, shotgun proteomics,Mycobacterium tuberculosis, Mycolicibacterium smegmatis, posttranslation modification, LC-MS/MS |
Contact List
Karl Schmitz |
contact affiliation | Department of Biological Sciences Department of Chemistry & Biochemistry University of Delaware Newark DE, 19716 |
contact email | schmitzk@udel.edu |
lab head | |
Emmanuel Ogbonna |
contact affiliation | University of Delaware |
contact email | eogbonna@udel.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032083
- Label: PRIDE project
- Name: Identification of arginine phosphorylation in Mycolicibacterium smegmatis by shotgun proteomics