PXD032078

PXD032078 is an original dataset announced via ProteomeXchange.

Title	Proteomics-based identification of ISG15 modification sites in vivo upon Coxsackie virus infection
Description	ISG15 is an interferon-stimulated, ubiquitin-like protein, with anti-viral activity against several clinically relevant viruses. However, its precise mechanism of action during viral infection remains elusive. Upon infection, ISG15 conjugates to protein substrates in covalent manner. We previously found that ISG15 restricts Coxsackie virus (CV) infection both in vitro and in vivo. Here, we endeavored to map the in vivo ISGylome following CV infection to mechanistically elucidate the function of ISG15 in host defense. To do so we combined a genetic approach employing a murine model of deregulated ISGylation with quantitative proteomics of immune-enriched endogenous ISG15 modification sites. In addition, we also quantified protein level changes in the host proteome following infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:00.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabien Thery
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Coxsackievirus; NCBI TaxID: NCBITaxon:12066;
ModificationList	ubiquitinylated lysine; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 05:25:12	ID requested
1	2024-05-23 00:39:47	announced
⏵ 2	2024-10-22 06:42:01	announced	2024-10-22: Updated project metadata.

10.1093/cvr/cvae026;

Bredow C, Thery F, Wirth EK, Ochs S, Kespohl M, Kleinau G, Kelm N, Gimber N, Schmoranzer J, Voss M, Klingel K, Spranger J, Renko K, Ralser M, M, ü, lleder M, Heuser A, Knobeloch KP, Scheerer P, Kirwan J, Br, ü, ning U, Berndt N, Impens F, Beling A, ISG15 blocks cardiac glycolysis and ensures sufficient mitochondrial energy production during Coxsackievirus B3 infection. Cardiovasc Res, 120(6):644-657(2024) [pubmed]

submitter keyword: Mouse, virus, ISG15, infection, ubiquitin

Francis Impens
contact affiliation	VIB Proteomics Core Ghent University VIB Proteomics Core VIB-UGent Center for Medical Biotechnology UGent Department of Biomolecular Medicine (lab head)
contact email	francis.impens@vib-ugent.be
lab head
Fabien Thery
contact affiliation	VIB-UGent
contact email	fabien.thery91@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD032078

PRIDE project URI

• PRIDE
  1. PXD032078
     1. Label: PRIDE project
     2. Name: Proteomics-based identification of ISG15 modification sites in vivo upon Coxsackie virus infection