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PXD032077

PXD032077 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImpact of IRP deficiency on the proteome of Ly6G+ cells from the bone marrow in mice
DescriptionThe overall objective of the project is to evaluate the effects of loss of function of iron regulatory proteins (IRP1, encoded by Aco1)-1 and -2 (IRP2, encoded by Ireb2) during adult life. Mice carrying floxed Aco1 and Ireb2 alleles were crossed to a knockin strain expressing a tamoxifen-inducible CRE recombinase under the control of the Rosa26 promoter. The resulting Aco1flox/flox,Ireb2flox/flox,Rosa26+/CreERT2 mice are designated P1/2-KO; littermates lacking the CreER sequence (Aco1flox/flox,Ireb2flox/flox,Rosa26+/+) serve as reference and are designated P1/2-CTR. Adult mice were injected (i.p.) with tamoxifen on day 1 and day 3 to ablate the IRPs in the whole body, and were sacrificed on day 10 for analysis. Bone marrow cells were collected and LY6G+ cells were magnetically sorted for proteome analysis by LC-MS/MS using an Ultimate 3000 UPLC system connected to an Orbitrap Exploris 480 mass spectrometer.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:41:06.330.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandro Altamura
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-05 05:24:53ID requested
12023-03-11 02:52:01announced
22023-11-14 08:41:08announced2023-11-14: Updated project metadata.
Publication List
Bonadonna M, Altamura S, Tybl E, Palais G, Qatato M, Polycarpou-Schwarz M, Schneider M, Kalk C, R, ü, diger W, Ertl A, Anstee N, Bogeska R, Helm D, Milsom MD, Galy B, Iron regulatory protein (IRP)-mediated iron homeostasis is critical for neutrophil development and differentiation in the bone marrow. Sci Adv, 8(40):eabq4469(2022) [pubmed]
Keyword List
submitter keyword: iron metabolism
neutrophils
granulopoiesis
hematopoiesis
iron regulatory proteins
iron responsive elements.
Contact List
Bruno Galy
contact affiliationDKFZ - German Cancer Research Center, “Division of Virus-Associated Carcinogenesis”, Im NeuenheimerFeld 280, 69120 Heidelberg, Germany
contact emailb.galy@dkfz-heidelberg.de
lab head
Sandro Altamura
contact affiliationMMPU - molecular medicine partnership unit
contact emailaltamura@embl.de
dataset submitter
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Dataset FTP location
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