PXD032077 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of IRP deficiency on the proteome of Ly6G+ cells from the bone marrow in mice |
Description | The overall objective of the project is to evaluate the effects of loss of function of iron regulatory proteins (IRP1, encoded by Aco1)-1 and -2 (IRP2, encoded by Ireb2) during adult life. Mice carrying floxed Aco1 and Ireb2 alleles were crossed to a knockin strain expressing a tamoxifen-inducible CRE recombinase under the control of the Rosa26 promoter. The resulting Aco1flox/flox,Ireb2flox/flox,Rosa26+/CreERT2 mice are designated P1/2-KO; littermates lacking the CreER sequence (Aco1flox/flox,Ireb2flox/flox,Rosa26+/+) serve as reference and are designated P1/2-CTR. Adult mice were injected (i.p.) with tamoxifen on day 1 and day 3 to ablate the IRPs in the whole body, and were sacrificed on day 10 for analysis. Bone marrow cells were collected and LY6G+ cells were magnetically sorted for proteome analysis by LC-MS/MS using an Ultimate 3000 UPLC system connected to an Orbitrap Exploris 480 mass spectrometer. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:41:06.330.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandro Altamura |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-05 05:24:53 | ID requested | |
1 | 2023-03-11 02:52:01 | announced | |
⏵ 2 | 2023-11-14 08:41:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bonadonna M, Altamura S, Tybl E, Palais G, Qatato M, Polycarpou-Schwarz M, Schneider M, Kalk C, R, ü, diger W, Ertl A, Anstee N, Bogeska R, Helm D, Milsom MD, Galy B, Iron regulatory protein (IRP)-mediated iron homeostasis is critical for neutrophil development and differentiation in the bone marrow. Sci Adv, 8(40):eabq4469(2022) [pubmed] |
Keyword List
submitter keyword: iron metabolism |
neutrophils |
granulopoiesis |
hematopoiesis |
iron regulatory proteins |
iron responsive elements. |
Contact List
Bruno Galy |
contact affiliation | DKFZ - German Cancer Research Center, “Division of Virus-Associated Carcinogenesis”, Im NeuenheimerFeld 280, 69120 Heidelberg, Germany |
contact email | b.galy@dkfz-heidelberg.de |
lab head | |
Sandro Altamura |
contact affiliation | MMPU - molecular medicine partnership unit |
contact email | altamura@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032077
- Label: PRIDE project
- Name: Impact of IRP deficiency on the proteome of Ly6G+ cells from the bone marrow in mice