PXD032071

PXD032071 is an original dataset announced via ProteomeXchange.

Title	Lung fibroblasts from chinese hamster cell line V79 and the effects of e-cigarettes liquids
Description	Although still considered a safer alternative to classical cigarettes, growing body of work point to harmfull effects of e-cigarettes affecting range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylations and direct oxidative modifications, was observed. Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e- cigarettes health impact.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:00:26.574.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032071
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarina Smiljanic
SpeciesList	scientific name: Cricetulus griseus; NCBI TaxID: 10029;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-05 05:15:01	ID requested
⏵ 1	2022-08-12 00:00:27	announced

Trifunovic S, Smiljani, ć K, Sickmann A, Solari FA, Kolarevic S, Divac Rankov A, Ljujic M, Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. Respir Res, 23(1):191(2022) [pubmed]

submitter keyword: e-cigarettes liquid, nicotine, V79, lung fibroblast, posttranslational modifications, metabolic cooperation

Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Bunsen-Kirchhoff-Straße 11, Dortmund, Germany. Medizinische Fakultät, Medizinisches Proteom-Center (MPC), Ruhr-Universität Bochum, 44801 Bochum, Germany. Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen AB243FX Scotland, UK.
contact email	albert.sickmann@isas.de
lab head
Katarina Smiljanic
contact affiliation	Senior Research Associate at University of Belgrade-Faculty of Chemistry, Center of Excellence for Molecular Food Sciences
contact email	katarinas@chem.bg.ac.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032071
     1. Label: PRIDE project
     2. Name: Lung fibroblasts from chinese hamster cell line V79 and the effects of e-cigarettes liquids