PXD032063

PXD032063 is an original dataset announced via ProteomeXchange.

Title	Detecting mistranslation from a serine tRNA with proline anticodon expressed in yeast
Description	Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to detect and quantify mistranslation from a serine tRNA variant with proline UGG anticodon and G26A secondary mutation engineered in yeast
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:01:24.563.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Berg
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-04 09:32:23	ID requested
⏵ 1	2022-08-12 00:01:25	announced

Berg MD, Zhu Y, Loll-Krippleber R, San Luis BJ, Genereaux J, Boone C, Vill, é, n J, Brown GW, Brandl CJ, Genetic background and mistranslation frequency determine the impact of mistranslating tRNASerUGG. G3 (Bethesda), 12(7):(2022) [pubmed]

submitter keyword: Yeast, mistranslation, tRNA

Judit Villen
contact affiliation	Department of Genome Sciences, University of Washington, Seattle, USA
contact email	jvillen@uw.edu
lab head
Matthew Berg
contact affiliation	Department of Genome Sciences, University of Washington
contact email	mattberg@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032063
     1. Label: PRIDE project
     2. Name: Detecting mistranslation from a serine tRNA with proline anticodon expressed in yeast