PXD032060

PXD032060 is an original dataset announced via ProteomeXchange.

Title	Optimization of Elution Conditions for Biotinylated Peptides from Tamavidin 2-REV
Description	We attempted to optimize the conditions for competitive elution of biotinylated peptides from the Tamavidin 2-REV beads. First, to optimize the temperature at which biotinylated peptides were eluted from the beads, biotinylated peptides were eluted with the biotin solution at 4, 37, 56, and 95 ºC. Second, we investigated whether the contamination of non-biotinylated peptides in the eluates could be reduced by adding a step of ‘mock’ elution with biotin-free buffer immediately before elution with the biotin solution. After the beads were incubated with a biotin-free buffer for 15 min at 37 ºC, the buffer was removed, and biotinylated peptides were eluted from the beads with the biotin solution at 37 ºC. Third, we compared biotin solutions containing various concentrations of NaCl (150, 250, 500, 1000, 2000 and 4000 mM). Finally, biotinylated peptides enriched by the original and optimized workflows from 300 µg proteins of RAW264.7 cells expressing TurboID-STING were compared by LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2023-03-04
AnnouncementXML	Submission_2023-03-03_07:00:16.437.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kohei Nishino
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-biotinyl-L-lysine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-04 00:25:03	ID requested
⏵ 1	2023-03-03 07:00:16	announced

Dataset with its publication pending

submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, BioID

Hidetaka Kosako
lab head
Kohei Nishino
contact affiliation	Tokushima University
dataset submitter

jPOST dataset URI

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