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PXD032059

PXD032059 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReduction of Interference Ions Generated in the Enrichment Process of Biotinylated Peptides
DescriptionAfter tryptic digestion of proteins of RAW264.7 cells expressing TurboID-STING, biotinylated peptides were captured with Tamavidin 2-REV magnetic beads and eluted competitively with excess biotin. When the peptide samples were subjected to LC-MS/MS, high intensity peaks of interference and/or contaminant ions were observed at the MS1 level. To reduce interference ions, we compared the following conditions: (a) surfactants to solubilize proteins (RapiGest vs. PTS); (b) trypsin inactivation (Pefabloc SC vs. heat); (c) beads pre-wash (none vs. 10% ACN); and (d) biotin clean-up (none vs. GL-Tip SDB). To confirm that some contaminant ions were not derived from the digested peptides but from the biotin solution, Tamavidin 2-REV beads that had not been incubated with the digested peptides were eluted with the biotin solution or biotin-free solution, and the eluates were analyzed by LC-MS/MS.
HostingRepositoryjPOST
AnnounceDate2023-03-04
AnnouncementXMLSubmission_2023-03-03_07:00:11.471.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKohei Nishino
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxamidomethyl-L-cysteine; N6-biotinyl-L-lysine; L-methionine sulfoxide; alpha-amino acetylated residue; unknown modification
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-04 00:24:33ID requested
12023-03-03 07:00:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, BioID
Contact List
Hidetaka Kosako
lab head
Kohei Nishino
contact affiliationTokushima University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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