JIP4-mycDDK overexpressing SH-SY5Y cell treated with acrolein with or without Jak3 inhibitor VI. JIP4-mycDDK was partially purified with anti-flag M2 magnetic beads. After reduction and alkylation of the samples, trypsin/Lys-C digestion were performed.　Identification and quantification of the phosphorylation site of JIP4 by acrolein were analyzed using a mass spectrometer.