PXD032049

PXD032049 is an original dataset announced via ProteomeXchange.

Title	Proteomes of uropathogenic Escherichia coli growing in human urine and in J82 urinary bladder cell.
Description	Uropathogenic Escherichia coli (UPEC) are the most common cause of urinary tract infection (UTI). UPEC normally reside in the intestine, and during establishment of UTI, it undergoes metabolic adaptations, first to urine and then upon tissue invasion to the bladder cell interior. In order to understand these adaptations, we used quantitative proteomic profiling to characterize protein expression of UPEC strain UTI89 growing in human urine and when inside J82 bladder cells. In order to facilitate detection of UPEC proteins over the excess amount of eukaryotic proteins in bladder cells, we developed a method where proteins from UTI89 grown in MOPS and urine was spiked-in to enhance detection of bacterial proteins. More than 2000 E. coli proteins were detected. During growth in urine, proteins associated with iron acquisition and several amino acid uptake and biosynthesis systems, in particular arginine metabolism, were significantly upregulated. During growth in J82 cells, proteins related to iron uptake and arginine metabolisms were upregulated together with proteins involved in sulphur compound turnover. Results suggested that UPEC experience a richer environment in bladder cells compared to urine. There was no direct correlation between upregulated proteins and proteins reported to be essential for infections, showing that upregulation during growth does not signify that the proteins are essential for growth under a condition.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_10:10:16.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arkadiusz Nawrocki
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-03 12:21:46	ID requested
⏵ 1	2022-06-09 10:10:17	announced

Andersen S, Nawrocki A, Johansen AE, Herrero-Fresno A, Men, é, ndez VG, M, ø, ller-Jensen J, Olsen JE, Growing in Human Urine and in J82 Urinary Bladder Cells. Proteomes, 10(2):(2022) [pubmed]

submitter keyword: UPEC, Urinary tract infections, Proteome, Metabolism

Jakob Møller-Jensen
contact affiliation	Institut of Biochemistry and Molecular Biology, University of Southern Denmark
contact email	jakobm@bmb.sdu.dk
lab head
Arkadiusz Nawrocki
contact affiliation	University of Southern Denmark, Odense
contact email	arn@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032049
     1. Label: PRIDE project
     2. Name: Proteomes of uropathogenic Escherichia coli growing in human urine and in J82 urinary bladder cell.