PXD032046

PXD032046 is an original dataset announced via ProteomeXchange.

Title	Translational specialization in pluripotency by RBPMS poises future lineage-decisions
Description	Labeling active ribosome associated proteins by L-Azidohomoalanine (AHA)- pulldown. Humane embryonic stem cells were labeled via AHA in methionine-free medium for 40 min. AHA labeled nascent proteins together with ribosomes and associated proteins were isolated with beads. Samples were divided into ribosome plus associated protein fraction and nascent protein fraction by 8M UREA wash.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:16:30.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prerana Wagle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-03 12:06:19	ID requested
1	2023-10-26 00:12:59	announced
⏵ 2	2023-11-14 08:16:30	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: pluripotency,Active-ribosome associated proteins, exit of pluripotency, RBPMS

Leo Kurian
contact affiliation	1. Center for Molecular Medicine Cologne, University of Cologne, Robert-Koch-Str. 21, D- 50931 Cologne, Germany 2. Inst. for Neurophysiology, University of Cologne, Robert-Koch-Str. 39, D-50931 Cologne, Germany 3. CECAD; Cologne Cluster of Excellence in Cellular Stress Responses in Ageing-associated Diseases, University of Cologne, Joseph-Stelzmann-Str. 26, D-50931 Cologne, Germany
contact email	leo.kurian@uk-koeln.de
lab head
Prerana Wagle
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD032046

PRIDE project URI

• PRIDE
  1. PXD032046
     1. Label: PRIDE project
     2. Name: Translational specialization in pluripotency by RBPMS poises future lineage-decisions