PXD032043 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of black coral (Antipatharia) skeleton reveals hundreds of skeleton-associated proteins across two taxa |
Description | Black corals, ecologically important cnidarians found from shallow to deep ocean depths, form a strong yet flexible skeleton of sclerotized chitin and other biomolecules including proteins. The structure and mechanical properties of the chitin component of the skeleton have been well-characterized. However, the protein component has remained a mystery. Here we used liquid chromatography-tandem mass spectrometry to sequence proteins extracted from two species of common Red Sea black corals following either one or two cleaning steps. We detected hundreds of proteins between the two corals, nearly 70 of which are each others’ reciprocal best BLAST hit. Unlike stony corals, only a few of the detected proteins were moderately acidic (biased toward aspartic and/or glutamic acid residues) suggesting less of a role for these types of proteins in black coral skeleton formation as compared to stony corals. No distinct chitin binding domains were found in the proteins, but proteins annotated as having a role in protein and chitin modifications were detected. Our results support the integral role of proteins in black coral skeleton formation, structure, and function. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-05 |
AnnouncementXML | Submission_2022-06-05_10:56:57.700.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD032043 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | David Morgenstern |
SpeciesList | scientific name: Stichopathes; NCBI TaxID: 44170; scientific name: Stichopathes spiessi; NCBI TaxID: 44171; scientific name: Antipathes griggi; NCBI TaxID: 639590; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-03 12:03:46 | ID requested | |
⏵ 1 | 2022-06-05 10:56:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: coral, skeleton, deep sea, bottom up |
Contact List
Tali Mass |
contact affiliation | Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mount Carmel, Haifa, 3498838, Israel |
contact email | tmass@univ.haifa.ac.il |
lab head | |
David Morgenstern |
contact affiliation | Weizmann institute |
contact email | david.morgenstern@weizmann.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032043
- Label: PRIDE project
- Name: Proteomic profiling of black coral (Antipatharia) skeleton reveals hundreds of skeleton-associated proteins across two taxa