PXD032043

PXD032043 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of black coral (Antipatharia) skeleton reveals hundreds of skeleton-associated proteins across two taxa
Description	Black corals, ecologically important cnidarians found from shallow to deep ocean depths, form a strong yet flexible skeleton of sclerotized chitin and other biomolecules including proteins. The structure and mechanical properties of the chitin component of the skeleton have been well-characterized. However, the protein component has remained a mystery. Here we used liquid chromatography-tandem mass spectrometry to sequence proteins extracted from two species of common Red Sea black corals following either one or two cleaning steps. We detected hundreds of proteins between the two corals, nearly 70 of which are each others’ reciprocal best BLAST hit. Unlike stony corals, only a few of the detected proteins were moderately acidic (biased toward aspartic and/or glutamic acid residues) suggesting less of a role for these types of proteins in black coral skeleton formation as compared to stony corals. No distinct chitin binding domains were found in the proteins, but proteins annotated as having a role in protein and chitin modifications were detected. Our results support the integral role of proteins in black coral skeleton formation, structure, and function.
HostingRepository	PRIDE
AnnounceDate	2022-06-05
AnnouncementXML	Submission_2022-06-05_10:56:57.700.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032043
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Morgenstern
SpeciesList	scientific name: Stichopathes; NCBI TaxID: 44170; scientific name: Stichopathes spiessi; NCBI TaxID: 44171; scientific name: Antipathes griggi; NCBI TaxID: 639590;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-03 12:03:46	ID requested
⏵ 1	2022-06-05 10:56:58	announced

Dataset with its publication pending

submitter keyword: coral, skeleton, deep sea, bottom up

Tali Mass
contact affiliation	Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mount Carmel, Haifa, 3498838, Israel
contact email	tmass@univ.haifa.ac.il
lab head
David Morgenstern
contact affiliation	Weizmann institute
contact email	david.morgenstern@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032043

PRIDE project URI

• PRIDE
  1. PXD032043
     1. Label: PRIDE project
     2. Name: Proteomic profiling of black coral (Antipatharia) skeleton reveals hundreds of skeleton-associated proteins across two taxa