PXD032035

PXD032035 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 ORF8 interactome in the secretory pathway
Description	The replication machinery of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is closely associated with the endoplasmic reticulum (ER) in host cells. Activation of the unfolded protein response (UPR) is a strategy hijacked by coronavirus to facilitate its replication and suppress host innate immunity. Here, we identified that many protein disulfide isomerase family members are enriched in the ORF8 interactome, and found that ORF8 protein accumulates in the ER and escapes the degradation system by forming mixed disulfide complexes with ER oxidoreductases. ORF8 induces the activation of three UPR pathways through targeting key UPR components, remodels ER morphology and accelerates protein trafficking.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:03:21.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jifeng Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-03 11:47:37	ID requested
⏵ 1	2022-08-12 00:03:22	announced

Liu P, Wang X, Sun Y, Zhao H, Cheng F, Wang J, Yang F, Hu J, Zhang H, Wang CC, Wang L, SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases. Redox Biol, 54():102388(2022) [pubmed]

submitter keyword: ORF8, 2-D gel, LC-MS/MS, endoplasmic reticulum

Wang Lei
contact affiliation	Institute of Biophysics,Chinese Academy of Sciences
contact email	wanglei@ibp.ac.cn
lab head
Jifeng Wang
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	516626982@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD032035

PRIDE project URI

• PRIDE
  1. PXD032035
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 ORF8 interactome in the secretory pathway