PXD032005

PXD032005 is an original dataset announced via ProteomeXchange.

Title	Identification of the endothelial cell microproteome - miPs in serum TASH patients
Description	Non-canonical small open reading frames (smORFs) that encode microproteins (miPs; <100 amino acids) are present in the genome. However, the study of smORF-encoded miPs has been hampered by technical challenges associated with the detection of small peptides by mass spectrometry. Here we combined deep RNA sequencing of ribosome-associated transcripts and optimized bioinformatic pipelines for downstream proteomic studies to identify thousands of smORF-derived miPs in endothelial cells (human 2,256, murine 7,597). Profound alterations in smORF/miP expression were detected following human endothelial cell activation by interleukin 1beat (IL-1beta) in vitro and by hypercholesterolemia and altered blood flow in vivo (in mice). Genome-wide homology analyses revealed that human smORFs are highly conserved among primates, and poorly in rodents or zebrafish. Functional characterization of selected miPs demonstrated their physical interaction with proteins in different subcellular compartments and their involvement in the regulation of proliferation, cell death, gene expression and inflammation. Moreover, it was possible to demonstrate that miPs can be released into the blood stream following cellular damage in humans. These findings demonstrate the value of this proteo-genomic pipeline for smORF and miP identification and highlight that the potential impact of miPs on cell and tissue function is much larger than previously presumed.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-24_02:38:33.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Graumann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 09:02:02	ID requested
⏵ 1	2026-08-24 02:38:34	announced

10.1093/cvr/cvag110;

Siragusa M, Graumann J, Kuenne C, G, ü, nther S, Jeratsch S, G, ü, ven B, S, ü, sser S, Talyan S, Bezuneh B, Matthess Y, Cartura M, Zhou X, Cho H, Sachs N, Maegdefessel L, M, ü, ller OJ, Troidl C, Nef HM, Looso M, Kaulich M, Offermanns S, Fleming I, Identification of unannotated microproteins involved in endothelial cell homeostasis, dysfunction, and vascular disease. Cardiovasc Res, 122(9):1257-1270(2026) [pubmed]

submitter keyword: Small open reading frame, microprotein, endothelial cell

Mauro Siragusa
contact affiliation	Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Frankfurt am Main, Germany.
contact email	siragusa@vrc.uni-frankfurt.de
lab head
Johannes Graumann
contact affiliation	Institute of Translational Proteomics, Faculty of Medicine, Philipps-Universität Marburg
contact email	johannes.graumann@uni-marburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032005
     1. Label: PRIDE project
     2. Name: Identification of the endothelial cell microproteome - miPs in serum TASH patients