PXD032003

PXD032003 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CT26 Proteome and Immunopeptidome treated with Ionizing Radiation
Description	This project explored the relationship between ionizing radiation and antigen presentation using both proteomic and immunopeptidomics methodologies. We investigated the radiation-specific changes which occur in the colorectal tumor cell proteome associated with changes in dose and time after irradiation. We used this data to observe the changes in key regulators and effector proteins of the antigen processing and presentation machinery. Furthermore, a parallel immunopeptidomics analysis enabled a peptide-level assessment of antigen presentation to correlate changes observed in the proteome thereby defining a radiation-specific peptide repertoire. A nuanced relationship between protein expression and antigen presentation was observed where radiation-induced changes in proteins do not always correlate with increased presentation of associated peptides. Furthermore, a neoantigen which increases in the context of radiation was characterised. This study provides new insights into how radiation enhances antigen processing and presentation that could be suitable for the development of combinatorial therapies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:03:32.906.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032003
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arun Tailor
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 08:17:25	ID requested
1	2022-10-04 01:05:22	announced
⏵ 2	2023-11-14 08:03:38	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD032003;
Tailor A, Estephan H, Parker R, Woodhouse I, Abdulghani M, Nicastri A, Jones K, Salatino S, Muschel R, Humphrey T, Giaccia A, Ternette N, Ionizing Radiation Drives Key Regulators of Antigen Presentation and a Global Expansion of the Immunopeptidome. Mol Cell Proteomics, 21(11):100410(2022) [pubmed]

10.6019/PXD032003;

Tailor A, Estephan H, Parker R, Woodhouse I, Abdulghani M, Nicastri A, Jones K, Salatino S, Muschel R, Humphrey T, Giaccia A, Ternette N, Ionizing Radiation Drives Key Regulators of Antigen Presentation and a Global Expansion of the Immunopeptidome. Mol Cell Proteomics, 21(11):100410(2022) [pubmed]

Keyword List

submitter keyword: interferon signaling, ligandomics, MHC, ionizing radiation,immunopeptidomics

submitter keyword: interferon signaling, ligandomics, MHC, ionizing radiation,immunopeptidomics

Contact List

Nicola Ternette
contact affiliation	Jenner Institute Antigen Discovery Group University of Oxford
contact email	nicola.ternette@ndm.ox.ac.uk
lab head
Arun Tailor
contact affiliation	University of Oxford
contact email	arun.tailor@ndm.ox.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032003

PRIDE project URI

Repository Record List

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