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PXD031992

PXD031992 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential UBE2H-CTLH E2-E3 ubiquitylation modulesregulate erythroid maturation
DescriptionThe developmentof haematopoietic stem cellsinto mature erythrocytes–erythropoiesis –is a controlled process characterized by cellular reorganisation and drastic reshaping of the proteome landscape.Failure of ordered erythropoiesis isassociated with anaemias and haematological malignancies. Although the ubiquitin (UB) system isa known crucial post-translational regulator in erythropoiesis, how the erythrocyteis reshaped by the UBsystemis poorly understood.Bymeasuringthe proteomiclandscape of in vitrohuman erythropoiesis models, we founddynamic differential expression ofsubunitsof the CTLH E3 ubiquitin ligase complexthat formeddistinctmaturation stage-dependent assemblies of structurally homologous RANBP9-and RANBP10-CTLH complexes.Moreover,protein abundance of CTLH’s cognate E2-conjugating enzyme UBE2H increased during terminal differentiation,which dependedon catalyticallyactive CTLH E3complexes.CRISPR-Cas9 mediated inactivation of all CTLH E3 assemblies by targeting the catalytic subunit MAEA,orUBE2H, triggered spontaneous and accelerated maturationof erythroid progenitor cellsincluding increased heme and haemoglobin synthesis. Thus, the orderly progression of human erythropoiesis is controlled by the assembly of distinct UBE2H-CTLHmodulesfunctioning at different developmental stages.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:42:45.123.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-02 01:34:47ID requested
12023-03-11 08:12:41announced
22023-11-14 08:42:47announced2023-11-14: Updated project metadata.
Publication List
Sherpa D, Mueller J, Karayel Ö, Xu P, Yao Y, Chrustowicz J, Gottemukkala KV, Baumann C, Gross A, Czarnecki O, Zhang W, Gu J, Nilvebrant J, Sidhu SS, Murray PJ, Mann M, Weiss MJ, Schulman BA, Alpi AF, Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation. Elife, 11():(2022) [pubmed]
Keyword List
submitter keyword: UBE2H, proteomics, DIA, MS, Gid complex, ubiquitination, E2, CTLH complex, E3
Contact List
Matthias Mann
contact affiliationProteomics and Signal Transduction Department Max Planck Institute of Biochemistry
contact emailmmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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