PXD031992 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential UBE2H-CTLH E2-E3 ubiquitylation modulesregulate erythroid maturation |
Description | The developmentof haematopoietic stem cellsinto mature erythrocytes–erythropoiesis –is a controlled process characterized by cellular reorganisation and drastic reshaping of the proteome landscape.Failure of ordered erythropoiesis isassociated with anaemias and haematological malignancies. Although the ubiquitin (UB) system isa known crucial post-translational regulator in erythropoiesis, how the erythrocyteis reshaped by the UBsystemis poorly understood.Bymeasuringthe proteomiclandscape of in vitrohuman erythropoiesis models, we founddynamic differential expression ofsubunitsof the CTLH E3 ubiquitin ligase complexthat formeddistinctmaturation stage-dependent assemblies of structurally homologous RANBP9-and RANBP10-CTLH complexes.Moreover,protein abundance of CTLH’s cognate E2-conjugating enzyme UBE2H increased during terminal differentiation,which dependedon catalyticallyactive CTLH E3complexes.CRISPR-Cas9 mediated inactivation of all CTLH E3 assemblies by targeting the catalytic subunit MAEA,orUBE2H, triggered spontaneous and accelerated maturationof erythroid progenitor cellsincluding increased heme and haemoglobin synthesis. Thus, the orderly progression of human erythropoiesis is controlled by the assembly of distinct UBE2H-CTLHmodulesfunctioning at different developmental stages. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:45.123.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-02 01:34:47 | ID requested | |
1 | 2023-03-11 08:12:41 | announced | |
⏵ 2 | 2023-11-14 08:42:47 | announced | 2023-11-14: Updated project metadata. |
Publication List
Sherpa D, Mueller J, Karayel Ö, Xu P, Yao Y, Chrustowicz J, Gottemukkala KV, Baumann C, Gross A, Czarnecki O, Zhang W, Gu J, Nilvebrant J, Sidhu SS, Murray PJ, Mann M, Weiss MJ, Schulman BA, Alpi AF, Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation. Elife, 11():(2022) [pubmed] |
Keyword List
submitter keyword: UBE2H, proteomics, DIA, MS, Gid complex, ubiquitination, E2, CTLH complex, E3 |
Contact List
Matthias Mann |
contact affiliation | Proteomics and Signal Transduction Department Max Planck Institute of Biochemistry |
contact email | mmann@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031992 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031992
- Label: PRIDE project
- Name: Differential UBE2H-CTLH E2-E3 ubiquitylation modulesregulate erythroid maturation