PXD031985

PXD031985 is an original dataset announced via ProteomeXchange.

Title	Conformational rearrangements upon start codon recognition in human 48S translation initiation complex
Description	Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct ribosome populations formed on an mRNA with a cognate AUG codon in the presence of eIF1A, eIF1, eIF2–GTP–Met-tRNAiMet, eIF3, eIF4A and eIF4B. The ‘open’ 40S subunit conformation differs from the human 48S scanning complex and represents an intermediate preceding the codon recognition step. The ‘closed’ form is similar to reported structures of complexes from yeast and mammals formed upon codon recognition, except for the orientation of eIF1A, which is unique in our structure. Kinetic experiments show how various initiation factors mediate the population distribution of open and closed conformations until 60S subunit docking. Our results provide insights into the timing and structure of human translation initiation intermediates and suggest the differences in the mechanisms of start codon selection between mammals and yeast.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_08:59:38.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Urlaub
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 01:10:57	ID requested
⏵ 1	2022-06-09 08:59:40	announced

Yi SH, Petrychenko V, Schliep JE, Goyal A, Linden A, Chari A, Urlaub H, Stark H, Rodnina MV, Adio S, Fischer N, Conformational rearrangements upon start codon recognition in human 48S translation initiation complex. Nucleic Acids Res, 50(9):5282-5298(2022) [pubmed]

submitter keyword: Ribosome, 48S initiation comples, eIF1

Prof. Dr. Henning Urlaub
contact affiliation	Bioanalytical Mass spectroscopy Group, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Bioanalytics, Institute for Clinical Chemistry, University Medical Center Göttingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Henning Urlaub
contact affiliation	Max-Plank Institute for Biophysical Chemistry
contact email	msbio.goettingen@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031985
     1. Label: PRIDE project
     2. Name: Conformational rearrangements upon start codon recognition in human 48S translation initiation complex