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PXD031984

PXD031984 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSingle-copy locus isolation identifies distinct proteomes of early- and late-firing DNA replication origin chromatin
DescriptionThe chromatin at origins of replication is the template for DNA replication initiation in eukaryotic genomes. However, whether the chromatin composition instructs individual origins to fire early-efficiently (EE) or late-inefficiently (LI) remains to be investigated. Here, we used site-specific recombination and a single-locus chromatin isolation approach to purify selected EE and LI replication origins from chromosome III of Saccharomyces cerevisiae. Using mass spectrometry, we define the histone modification landscape and identify the protein composition of defined ~1kb native chromatin regions surrounding the EE and LI replication start sites. We confirm expected origin interactors but also find unexpected interactions, such as the Ask1 subunit of the kinetochore-associated DASH complex. Strikingly, we show that Ask1 is a critical factor in the replication timing control of specific origins in yeast. These results highlight that our unbiased approach can specifically identify the functionally-relevant proteome at a single-copy locus of interest and provide a detailed view of histone modification and protein interaction networks on replication origin chromatin. The current dataset contains proteomic data used for the histone PTM analysis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:45:26.070.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrey Tvardovskiy
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonomethylated residue; acetylated residue; dimethylated residue; N6,N6,N6-trimethyl-L-lysine
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-02 01:10:39ID requested
12024-10-07 13:45:35announced
22024-10-22 05:45:28announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2023.112045;
Wei, β M, Chanou A, Schauer T, Tvardovskiy A, Meiser S, K, ö, nig AC, Schmidt T, Kruse E, Ummethum H, Trauner M, Werner M, Lalonde M, Hauck SM, Scialdone A, Hamperl S, Single-copy locus proteomics of early- and late-firing DNA replication origins identifies a role of Ask1/DASH complex in replication timing control. Cell Rep, 42(2):112045(2023) [pubmed]
Keyword List
submitter keyword: replication timing, replication efficiency, Affinity purification,Locus-specific chromatin isolation, DNA replication origin, histone PTMs, Site-specific recombination
Contact List
Stephan Hamperl
contact affiliationChromosome dynamics and genome stability, Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, Feodor-Lynen-Strasse 21, 81377 München, Germany
contact emailstephan.hamperl@helmholtz-muenchen.de
lab head
Andrey Tvardovskiy
contact affiliationInstitute of Functional Epigenetics, Helmholtz Zentrum München, Neuherberg 85764, Germany
contact emailandrey.tvardovskiy@helmholtz-muenchen.de
dataset submitter
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Dataset FTP location
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