PXD031982

PXD031982 is an original dataset announced via ProteomeXchange.

Title	Isolation of interstitial fluids reveals novel secreted bioactive proteins from muscle and fat tissues
Description	Proteins are secreted from various cells to send information to neighboring cells or distant tissues. Because of the highly integrated nature of energy balance systems, there has been a great deal of interest in myokines and adipokines, proteins secreted from muscle and fat tissues, respectively. These have been challenging to study through proteomics because serum is loaded with super-abundant proteins that limit the detection of proteins that have a low, hormone-like abundance. We show here that interstitial fluids (IFs) can be harvested easily from muscle and fat tissues of mice by very low-speed centrifugation and these fluids show a very different protein constellation than that of plasma or tissues. Under several different perturbations in vivo, like exercise or cold, quantitative Mass Spectrometry of these IFs allowed for the identification of many novel myokines and adipokines, including factors both increased and decreased in abundance. Finally, we identify prosaposin, a well-known neurotrophic factor, as a secreted product of both muscle and fat tissues; recombinant prosaposin stimulates a gene program related to thermogenesis in primary fat cells, indicating one potential function for PSAP.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:40:34.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Van Vranken
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 01:09:50	ID requested
1	2023-02-10 09:47:15	announced
⏵ 2	2023-11-14 07:40:38	announced	2023-11-14: Updated project metadata.

Mittenb, ü, hler MJ, Jedrychowski MP, Van Vranken JG, Sprenger HG, Wilensky S, Dumesic PA, Sun Y, Tartaglia A, Bogoslavski D, A M, Xiao H, Blackmore KA, Reddy A, Gygi SP, Chouchani ET, Spiegelman BM, Isolation of extracellular fluids reveals novel secreted bioactive proteins from muscle and fat tissues. Cell Metab, 35(3):535-549.e7(2023) [pubmed]

submitter keyword: Serum Proteomics, Interstitial fluid proteomics

Bruce Spiegelman
contact affiliation	Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
contact email	bruce_spiegelman@dfci.harvard.edu
lab head
Jonathan Van Vranken
contact affiliation	Harvard Medical School
contact email	van@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD031982

PRIDE project URI

• PRIDE
  1. PXD031982
     1. Label: PRIDE project
     2. Name: Isolation of interstitial fluids reveals novel secreted bioactive proteins from muscle and fat tissues