PXD031981

PXD031981 is an original dataset announced via ProteomeXchange.

Title	Phytopathogenic protist Albugo candida secretes cationic intrinsically disordered peptides with antimicrobial activity against plant-associated bacteria
Description	Positive and negative ecological interactions shape the dynamics and composition of natural microbial communities. The mechanisms behind microbe-microbe interactions, particularly those protein-based, are not well understood and only a small percentage of such interactions has been studied. We hypothesize that secreted proteins are a powerful and highly specific toolset to shape and defend a favorable plant niche. Here, we have studied Albugo candida, an obligate plant parasite from the protist Oomycota phylum, for its potential to inhibit and promote the growth of bacteria through secretion of proteins into the apoplast. Amplicon sequencing and network analysis of Albugo-infected and uninfected samples revealed an abundance of negative correlations between Albugo and other phyllosphere microbes. Analysis of the secreted proteome of Albugo candida combined with machine-learning predictors enabled the selection of candidates for heterologous expression and study of their inhibitory activity in vitro. We found that three of the candidate proteins showed a selective antimicrobial activity on several gram-positive bacterial strains isolated from Arabidopsis thaliana. We could ascribe the antibacterial activity of the candidates to their intrinsically disordered regions and positively correlate it with their net charge. This is the first report of protist proteins that have an antimicrobial activity under apoplastic conditions and therefore are potential biocontrol tools for a targeted manipulation of the microbiome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:46:02.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Gomez Perez
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Albugo laibachii Nc14; NCBI TaxID: 890382; scientific name: Albugo candida AcNc2; NCBI TaxID: 1204551;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-02 01:09:31	ID requested
1	2023-05-31 04:18:46	announced
2	2023-11-14 08:55:54	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:46:03	announced	2024-10-22: Updated project metadata.

10.1111/NPH.18995;

submitter keyword: Albugo,Arabidopsis, Apoplast

Eric Kemen
contact affiliation	Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany
contact email	eric.kemen@uni-tuebingen.de
lab head
Daniel Gomez Perez
contact affiliation	University of Tuebingen
contact email	daniel.gomez-perez@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031981
     1. Label: PRIDE project
     2. Name: Phytopathogenic protist Albugo candida secretes cationic intrinsically disordered peptides with antimicrobial activity against plant-associated bacteria