PXD031978

PXD031978 is an original dataset announced via ProteomeXchange.

Title	Proteomic study on the reproductive toxicity of Tripterygium glycosides in rats
Description	Tripterygium glycoside tablet (TGT) is a common clinically used and effective non-steroidal immunosuppressant. However, its reproductive toxicity limits its application in pediatric immune diseases, warranting the study of the molecular mechanism behind its reproductive toxicity. In the present study, 4-week-old male SD mice were provided TGT through continuous gavage with a clinically equivalent dose of 12 mg/kg for 12 weeks. The reproductive toxicity of TGT was recorded, and its toxicity mechanism was verified through experimental validation and proteomics analyses. Our results demonstrated that TGT could significantly reduce the testosterone level in the serum as well as the concentration and survival rate of sperms. Pathological sections of the testis revealed that TGT could reduce spermatocytes at different levels and make the convoluted meridians vacuolated. Based on TMT-labeled quantitative mice testicular tissue proteomics, 34 differential proteins were screened, involving protein digestion and absorption, PPAR signaling pathway, PI3K-Akt, and other pathways, among which PI3K-Akt plays an important role in the study of reproductive injury. Western blotting results revealed that TGT could significantly downregulate the Col1A1, Col1A2, p-PI3K, and p-Akt expressions and inhibit the expression of proteins related to the PI3K-Akt signaling pathway. In summary, the clinically equivalent dose of TGT induced reproductive toxicity of 4-week-old male SD mice, possibly in relation to the inhibition of the PI3K-Akt pathway expression.
HostingRepository	iProX
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2023-12-24_20:41:12.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanlin Dai
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-01 18:15:47	ID requested
1	2022-03-01 18:16:05	announced
⏵ 2	2023-12-24 20:41:14	announced	2023-12-25: Update information.

Dai Y, Sun L, Han S, Xu S, Wang L, Ding Y, Glycosides in Rats. Front Pharmacol, 13():888968(2022) [pubmed]

submitter keyword: proteomics reproductive toxicity, molecular mechanism, testis

Shanshan Han
contact affiliation	Henan University of Traditional Chinese Medicine
contact email	15093292627@163.com
lab head
Yanlin Dai
contact affiliation	Henan University of Traditional Chinese Medicine
contact email	dyl59140603@126.com
dataset submitter

iProX dataset URI