PXD031975

PXD031975 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling of mitotic arrest by transcriptional silencing of the Cdc20 homolog slp1 in fission yeast
Description	Depletion of the APC/C activator Cdc20 arrests cells in metaphase with high levels of the mitotic cyclin (Cyclin B) and the Separase inhibitor Securin. In mammalian cells this arrest has been exploited for the treatment of cancer with drugs that engage the spindle assembly checkpoint and, recently, with chemical inhibitors of the APC/C. While most cells arrested in mitosis for prolonged periods undergo apoptosis, others skip cytokinesis and enter G1 with unsegregated chromosomes. This process, known as mitotic slippage, generates aneuploidy and increases genomic instability in the cancer cell. Here, we analyse the behaviour of fission yeast cells arrested in mitosis through the transcriptional silencing of the Cdc20 homolog slp1. While depletion of slp1 readily halts cells in metaphase, this arrest is only transient and a majority of cells eventually undergo cytokinesis and show steady mitotic dephosphorylation. As a result, we generated this label-free dataset with the aim of providing further insights into the global dynamics of protein expression under slp1 depletion and the underlying mechanisms that contribute to the mitotic escape.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:30:15.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathalia Chica
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-01 10:25:14	ID requested
1	2022-10-13 11:56:39	announced
⏵ 2	2023-11-14 08:30:16	announced	2023-11-14: Updated project metadata.

Chica N, Portantier M, Nyquist-Andersen M, Espada-Burriel S, Lopez-Aviles S, Uncoupling of Mitosis and Cytokinesis Upon a Prolonged Arrest in Metaphase Is Influenced by Protein Phosphatases and Mitotic Transcription in Fission Yeast. Front Cell Dev Biol, 10():876810(2022) [pubmed]

submitter keyword: LC-MS, proteomics, fission yeast, label-free

Sandra Lopez-Aviles
contact affiliation	Centre for Molecular Medicine Norway (NCMM). School of Medicine. University of Oslo, Oslo, Norway. Institute of Biosciences (IBV). Faculty of Mathematics and Natural Sciences. University of Oslo, Oslo, Norway.
contact email	s.l.aviles@ncmm.uio.no
lab head
Nathalia Chica
contact affiliation	Department of Molecular Cell Biology, Institute for Cancer Research. The Norwegian Radium Hospital, Montebello, 0379. Oslo, Norway
contact email	nathac@uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031975

PRIDE project URI

• PRIDE
  1. PXD031975
     1. Label: PRIDE project
     2. Name: Proteomics profiling of mitotic arrest by transcriptional silencing of the Cdc20 homolog slp1 in fission yeast