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PXD031973

PXD031973 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of MACS isolated microglia cells (CD11b positive cells) in comparison to all non-CD11b positive cells (termed non-target cell fraction (NTCF)) with proteomics
DescriptionMicroglia isolation from adult mouse brains remains difficult in comparison to microglia isolation from brains from newborn mouse brains as connective tissue, extracellular matrix materials and thicker myelin sheaths substantially influence traditional isolation protocols used with newborn mouse brains. Since the introduction of magnetic activated cell sorting (MACS), it has been reported that it is possible to also obtain microglia cells from adult mouse brains with a very good purity. However, most studies only used flow cytometry and/or qPCR to determine microglia enrichment and enrichment of traditional microglia marker proteins. Therefore we wanted to characterize in an unbiased way the proteomes of with MACS isolated microglia cells (CD11b positive cells) in comparison to all non-CD11b positive cells (termed non-target cell fraction (NTCF)), which consist of primarily astrocytes, neurons and oligodendrocytes. Therefore, we isolated from three adult mouse brains via MACS CD11b positive cells as well as the remaining CD11b negative cells (NTCF) to characterize the proteome of both cell fractions. The obtained proteomes were compared to a dataset of proteomic signatures for all CNS cell types determined by Sharma et al., (2015, doi: 10.1038/nn.4160) to determine the amount of enrichment of microglia-specific proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:34:21.619.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmilio Cirri
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-01 10:24:18ID requested
12024-03-22 10:04:17announced
22024-10-22 06:34:22announced2024-10-22: Updated project metadata.
Publication List
10.3389/FIMMU.2024.1227355;
Keyword List
submitter keyword: CD11b positive cells, proteomics,microglia, NTCF
Contact List
Prof. Dr. med. habil. Reinhard Bauer
contact affiliationCMB - Center for Molecular Biomedicine Hans-Knöll-Str. 2 07745 Jena
contact emailReinhard.Bauer@med.uni-jena.de
lab head
Emilio Cirri
contact affiliationLeibniz Institute on Ageing Fritz Lipmann Institute Jena
contact emailemilio.cirri@fli-leibniz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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