PXD031966

PXD031966 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of MHC class I-associated peptidome derived from non-obese diabetic mouse thymus and pancreas
Description	The peptides repertoire presented to CD8+ T cells by major histocompatibility complex (MHC) class I molecules, referred to as the MHC I-associated peptidome (MIP), regulates all critical events that occur during the lifetime of CD8+ T cells. The MIP presented by thymic antigen presenting cells (APCs) is crucial for shaping CD8+ T cell repertoire and self-tolerance, while the MIP presented by peripheral tissues and organs is not only involved in maintaining periphery CD8+ T cell survival and homeostasis, but also mediates immune surveillance and autoimmune responses of CD8+ T cells under pathological conditions. Type 1 diabetes (T1D) is an organ-specific autoimmune disease caused by the destruction pancreatic β cells, mediated primarily by autoreactive CD8+ T cells. Non-obese diabetic (NOD) mouse is one of important animal models of spontaneous autoimmune diabetes that shares several key features with human T1D. Here, we deeply analyzed the MIP derived from the primary tissues of thymus and pancreas in NOD mice using targeted database searches of mass spectrometry data. We demonstrated that the thymus MIP source proteins accommodated only a small portion of the transcriptome of thymus epithelial cells, and partially shared with the MIP source proteins derived from NOD mice pancreas and β cell line. The global view of the MHC I-associated self-peptides repertoire in the thymus and pancreas of NOD mice may serve as a biological reference to identify potential autoantigens targeted by autoreactive CD8+ T cells in T1D.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:41:35.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lina wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-01 10:05:17	ID requested
1	2022-10-14 13:54:24	announced
⏵ 2	2023-11-14 07:41:37	announced	2023-11-14: Updated project metadata.

Wang L, Li X, Yang S, Chen X, Li J, Wang S, Zhang M, Zheng Z, Zhou J, Wang L, Wu Y, Proteomic identification of MHC class I-associated peptidome derived from non-obese diabetic mouse thymus and pancreas. J Proteomics, 270():104746(2023) [pubmed]

submitter keyword: pancreas, LC-MS/ MS, NOD mouse,MHC class I-associated peptidome, T1D, CD8+ T-cell., thymus

Yuzhang Wu
contact affiliation	Army Medical University (Third Military Medical University)
contact email	wuyuzhang@tmmu.edu.cn
lab head
lina wang
contact affiliation	no
contact email	1481752769@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031966
     1. Label: PRIDE project
     2. Name: Proteomic identification of MHC class I-associated peptidome derived from non-obese diabetic mouse thymus and pancreas