PXD031956

PXD031956 is an original dataset announced via ProteomeXchange.

Title	Host defesen effects of vitamin D
Description	To study the pleiotropic effects of vitamin D in airway host defense, we use airway epithelial organoids cultured on air-liquid interface (ALI), originating from three chronic rhinosinusitis patients, marking as N66, N67 and N69, respectively. 24 hours prior to infection of Staphylococcus Aureus or influenza virus H1N1, 10nM and 100nM 1,25(OH)2D3 were added respectively to the medium in the lower chamber of Transwells with organoids. 24 hours post infection, 100μl DPBS were added to the apical surface of organoids for 20 minutes and then cellular secretion diluted in the DPBS were collected for proteomics analysis. The control groups without 1,25(OH)2D3 treatment were denoted as ND and those without infection were denoted as C. We applied a mass spectrometry-based tandem mass tag quantitative proteomics to analyze the cellular secretion from airway epithelial organoids, so as to study the host defense effects of vitamin D against pathogen infection.
HostingRepository	iProX
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2024-03-20_00:11:15.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shumin Liao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 18:38:00	ID requested
⏵ 1	2024-03-20 00:11:16	announced

Liao S, Huang Y, Zhang J, Xiong Q, Chi M, Yang L, Zhang J, Li L, Fan Y, Vitamin D promotes epithelial tissue repair and host defense responses against influenza H1N1 virus and Staphylococcus aureus infections. Respir Res, 24(1):175(2023) [pubmed]

submitter keyword: influenza, airway epithelial organoids, vitaminD, proteomics

Liang li
contact affiliation	Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
contact email	liang.li@siat.ac.cn
lab head
Shumin Liao
contact affiliation	Department of Thoracic Surgery, The Seventh Affiliated Hospital Sun Yat-sen University
contact email	liaoshm7@mail.sysu.edu.cn
dataset submitter

iProX dataset URI