PXD031952 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | the role of neutrophil-produced collagen in skin injury |
Description | to assess the role of neutrophil-produced collagen in skin injury. 3 mouse groups (WT, Mrp8-Cre;TGFbR2-floxed and Mrp8-Cre;iDTR) were subjected to an ear injury by piercing. A limited area around the wound was dissected (6-10 mg tissue) and subjected to ECM extraction and TMT proteomics analysis. From each animal, an equivalent amount of skin from the other (healthy) ear was also excised and used as an internal control. 4 biological replicates per condition (WT, A and B) with 2 samples per animal (injured and healthy control) were distributed in three independent TMT 10-plex experiments. Samples from the same animal were kept together in the same TMT experiment. In each TMT experiment one of the channels was reserved for a common internal standard (I.S.) made by pooling the 24 peptide samples. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-28 |
AnnouncementXML | Submission_2025-02-28_02:04:37.295.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elena Bonzon-Kulichenko |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-28 12:25:09 | ID requested | |
⏵ 1 | 2025-02-28 02:04:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: neutrophil collagen hydroxilation inflammation |
Contact List
Jesús Vázquez Cobos |
contact affiliation | Cardiovascular Proteomics Laboratory & Proteomics Unit Vascular Pathophysiology Area Centro Nacional de Investigaciones Cardiovasculares Melchor Fernández Almagro núm. 3 28029 Madrid |
contact email | jvazquez@cnic.es |
lab head | |
Elena Bonzon-Kulichenko |
contact affiliation | University of Castilla-La Mancha |
contact email | elena.bonzon@uclm.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD031952 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031952
- Label: PRIDE project
- Name: the role of neutrophil-produced collagen in skin injury