PXD031952

PXD031952 is an original dataset announced via ProteomeXchange.

Title	the role of neutrophil-produced collagen in skin injury
Description	to assess the role of neutrophil-produced collagen in skin injury. 3 mouse groups (WT, Mrp8-Cre;TGFbR2-floxed and Mrp8-Cre;iDTR) were subjected to an ear injury by piercing. A limited area around the wound was dissected (6-10 mg tissue) and subjected to ECM extraction and TMT proteomics analysis. From each animal, an equivalent amount of skin from the other (healthy) ear was also excised and used as an internal control. 4 biological replicates per condition (WT, A and B) with 2 samples per animal (injured and healthy control) were distributed in three independent TMT 10-plex experiments. Samples from the same animal were kept together in the same TMT experiment. In each TMT experiment one of the channels was reserved for a common internal standard (I.S.) made by pooling the 24 peptide samples.
HostingRepository	PRIDE
AnnounceDate	2025-02-28
AnnouncementXML	Submission_2025-02-28_02:04:37.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Bonzon-Kulichenko
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 12:25:09	ID requested
⏵ 1	2025-02-28 02:04:37	announced

Dataset with its publication pending

submitter keyword: neutrophil collagen hydroxilation inflammation

Jesús Vázquez Cobos
contact affiliation	Cardiovascular Proteomics Laboratory & Proteomics Unit Vascular Pathophysiology Area Centro Nacional de Investigaciones Cardiovasculares Melchor Fernández Almagro núm. 3 28029 Madrid
contact email	jvazquez@cnic.es
lab head
Elena Bonzon-Kulichenko
contact affiliation	University of Castilla-La Mancha
contact email	elena.bonzon@uclm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD031952

PRIDE project URI

• PRIDE
  1. PXD031952
     1. Label: PRIDE project
     2. Name: the role of neutrophil-produced collagen in skin injury