PXD031920

PXD031920 is an original dataset announced via ProteomeXchange.

Title	Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis
Description	Centrosomes are orbited by centriolar satellites, dynamic multiprotein assemblies nucleated by PCM1. To study the requirement for centriolar satellites, we generated mice lacking PCM1. Pcm1-/- mice display partially expressive perinatal lethality with survivors exhibiting hydrocephalus, oligospermia and cerebellar hypoplasia. Pcm1-/- multiciliated ependymal cells and PCM1-/- retinal pigmented epithelial 1 (RPE1) cells showed reduced ciliogenesis. PCM1-/- RPE1 cells displayed reduced docking of the mother centriole to the ciliary vesicle and removal of CP110 and CEP97 from the distal mother centriole, indicating compromised early ciliogenesis. We show these molecular cascades are maintained in vivo, although we propose that the cellular threshold for ciliogenesis initiation varies between cell types. We propose that PCM1 and centriolar satellites facilitate efficient trafficking of proteins to and from centrioles, including the departure of CP110 and CEP97 to initiate ciliogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:05.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-28 02:49:00	ID requested
1	2023-05-10 13:52:30	announced
⏵ 2	2023-11-14 08:47:06	announced	2023-11-14: Updated project metadata.

Hall EA, Kumar D, Prosser SL, Yeyati PL, Herranz-P, é, rez V, Garc, í, a-Verdugo JM, Rose L, McKie L, Dodd DO, Tennant PA, Megaw R, Murphy LC, Ferreira MF, Grimes G, Williams L, Quidwai T, Pelletier L, Reiter JF, Mill P, Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis. Elife, 12():(2023) [pubmed]

submitter keyword: MTECs,Cilia

Alex von Kriegsheim
contact affiliation	university of edinburgh
contact email	alex.vonkriegsheim@ied.ac.uk
lab head
Alex von kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD031920

PRIDE project URI

• PRIDE
  1. PXD031920
     1. Label: PRIDE project
     2. Name: Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis