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PXD031898

PXD031898 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmmonium Sulfate-based Prefractionation Improved Proteome Coverage in Arabidopsis thaliana Leaf Extract
DescriptionThe hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed, of which the results validated this hypothesis. Of note, this prefractionation method allowed to identify 45% more proteins compared to the number of proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry drastically improved the detection of intact and post-translationally modified proteins.
HostingRepositoryMassIVE
AnnounceDate2023-02-19
AnnouncementXMLSubmission_2023-02-19_10:42:08.429.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTagnon Missihoun
SpeciesList scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-25 11:58:06ID requested
12023-02-19 10:42:08announced
Publication List
no publication
Keyword List
submitter keyword: Arabidopsis thaliana, Ammonium sulafte-based prefractionation, Plant leaves, Orbitrap Fusion, Proteome offline prefractionation
Contact List
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
lab head
Tagnon Missihoun
contact affiliationUniversity of Quebec Trois-Rivieres
contact emailTagnon.Missihoun@uqtr.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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