PXD031887

PXD031887 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Whole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells
Description	A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we characterise this knockout strain by quantitative whole cell proteomics. Additionally, we analyse the way ISG75 knockout cells respond to suramin on the proteome level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:20.850.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 10:28:25	ID requested
1	2023-03-11 03:43:18	announced
⏵ 2	2023-11-14 08:42:22	announced	2023-11-14: Updated project metadata.

Publication List

Makarov A, Began J, Mautone IC, Pinto E, Ferguson L, Zoltner M, Zoll S, Field MC, The role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes. Microb Cell, 10(2):18-35(2023) [pubmed]

Makarov A, Began J, Mautone IC, Pinto E, Ferguson L, Zoltner M, Zoll S, Field MC, The role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes. Microb Cell, 10(2):18-35(2023) [pubmed]

Keyword List

submitter keyword: xenobiotics, drug metabolism, drug accumulation, trypanosome, CRISPR/Cas9,invariant surface glycoprotein, suramin

submitter keyword: xenobiotics, drug metabolism, drug accumulation, trypanosome, CRISPR/Cas9,invariant surface glycoprotein, suramin

Contact List

Mark C. Field
contact affiliation	Biological Chemistry and Drug Discovery Wellcome Centre for Anti-Infectives Research (WCAIR) School of Life Sciences University of Dundee DUNDEE DD15EH UK
contact email	mfield@mac.com
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031887
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031887

PRIDE project URI

Repository Record List

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