PXD031875

PXD031875 is an original dataset announced via ProteomeXchange.

Title	Biomarkers for canine Leptospira vaccine potency
Description	Mandatory potency testing of Leptospira vaccine batches relies partially on in vivo procedures, requiring large numbers of laboratory animals. Cell-based assays could replace in vivo tests if biomarkers indicative of Leptospira vaccine potency are identified. We investigated innate immune responsiveness induced by inactivated L. interrogans serogroups Canicola and Icterohaemorrhagiae, and two bivalent, non-adjuvanted canine Leptospira vaccines containing the same serogroups. First, the transcriptome and proteome analysis of canine 030-D cells stimulated with Leptospira strains, and the corresponding vaccine revealed more than 900 DEGs and 23 DEPs in common to these three stimuli. Second, comparison of responses induced by this Leptospira vaccine and a vaccine from another manufacturer revealed a large overlap in DEGs and DEPs as well, suggesting potential to identify biomarkers of Leptospira vaccine activity. Because not many common DEPs were identified, we selected seven molecules from the identified DEGs, associated with pathways related to innate immunity, of which CXCL-10, IL-1β, SAA, and complement C3 showed increased secretion upon stimulation with both Leptospira vaccines. These molecules could be interesting targets for development of biomarker-based assays in the future. Additionally, this study contributes to the understanding of the mechanisms by which Leptospira vaccines induce innate immune responses in the dog.
HostingRepository	PRIDE
AnnounceDate	2022-03-06
AnnouncementXML	Submission_2022-03-06_07:00:14.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreja Novak
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 10:12:45	ID requested
⏵ 1	2022-03-06 07:00:15	announced

Dataset with its publication pending

submitter keyword: Leptospira, dog, monocyte, biomarker, innate immunity, vaccine

Arjen Sloots
contact affiliation	Intravacc, Bilthoven, the Netherlands
contact email	arjen.sloots@intravacc.nl
lab head
Andreja Novak
contact affiliation	Utrecht University
contact email	a.novak@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031875
     1. Label: PRIDE project
     2. Name: Biomarkers for canine Leptospira vaccine potency