PXD031874

PXD031874 is an original dataset announced via ProteomeXchange.

Title	Trafficking and interactions upon loss of TECPR2
Description	Hereditary sensory and autonomic neuropathy 9 (HSAN9) is a rare neurological disease caused by mutations in the gene encoding for Tectonin β-propeller repeat containing protein 2 (TECPR2) which possibly result in loss of the protein. Beside its potential role in autophagy, TECPR2 may serve as positive modulator of COPII-mediated ER export. However, the molecular consequences of TECPR2 deficiency for the secretory pathway remain unclear, in particular with regard to specific cargo proteins. By employing spatial proteomic approaches, we unveiled pronounced changes in several steps along the secretory pathway with numerous proteins important for neuronal function being affected in their intracellular trafficking or secretion upon loss of TECPR2. Moreover, TECPR2’s associations with endomembranes and components of the secretory pathway were strongly reduced when a HSAN9 mutant TECPR2 variant was expressed. Collectively, this resource provides the first systemic profiling of global secretory pathway defects in a loss of function HSAN9 cell model.
HostingRepository	PRIDE
AnnounceDate	2023-01-09
AnnouncementXML	Submission_2023-01-09_03:12:19.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChristianBehrends
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 10:12:23	ID requested
⏵ 1	2023-01-09 03:12:20	announced

Dataset with its publication pending

submitter keyword: TECPR2, COPII, APEX2, proximity biotinylation, LysoIP, ERES, RUSH SPECS, SUSPECS,HSAN9, SPG49

ChristianBehrends
contact affiliation	Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-University München, Munich, Germany
contact email	christian.behrends@mail03.med.uni-muenchen.de
lab head
ChristianBehrends
contact affiliation	SyNergy, LMU München
contact email	christian.behrends@mail03.med.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031874
     1. Label: PRIDE project
     2. Name: Trafficking and interactions upon loss of TECPR2