PXD031870 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BioID proximity labelling of MAP4K4 interactors in DAOY medulloblastoma cells |
Description | Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) is Ser/Thr protein kinase highly expressed in medulloblastoma (MB) patient samples which promotes cell migration and invasiveness downstream of growth factor receptors. Although MAP4K4 implication in the control of the membrane protrusions at the leading edge of cells during migration and invasion in MB is established, its downstream effectors governing and mediating this process in MB have not been identified. In this study we explored the proteomic neighborhood of MAP4K4 in MB cells and to identify candidate potentially affecting its activity and pro-migratory functions. Towards this aim, we expressed BioID fused versions of MAP4K4 (N- and C-terminal) in DAOY cells under presence of biotin. In the control setup, we expressed the BioID enzyme alone. After Streptavidin purification of biotinylated proteins, we performed mass spectrometry and quantified relative abundances compared to control conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-04 |
AnnouncementXML | Submission_2022-08-04_02:07:17.249.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD031870 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Martin Baumgartner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-24 10:02:15 | ID requested | |
⏵ 1 | 2022-08-04 02:07:17 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: BioID, MAP4K4, medulloblastoma |
Contact List
Martin Baumgartner |
contact affiliation | Pediatric Molecular Neuro-Oncology Research, Department of Oncology, University Children's Hospital Zurich |
contact email | Martin.Baumgartner@kispi.uzh.ch |
lab head | |
Martin Baumgartner |
contact affiliation | University Children's Hospital Zürich |
contact email | Martin.Baumgartner@kispi.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031870 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031870
- Label: PRIDE project
- Name: BioID proximity labelling of MAP4K4 interactors in DAOY medulloblastoma cells