PXD031869

PXD031869 is an original dataset announced via ProteomeXchange.

Title	PT-modified B12 probe and Non-PT-modified B34 probe pull-down with Salmonella enterica serovar Cerro 87 cell lysate
Description	To capture proteins that exhibit differential binding with PT- and non-PT-modified DNA in vivo, we performed pull-down assays using biotinylated DNA probes and the cell lysate of S. enterica serovar Cerro 87 that contains the dndBCDE-dndFGH module. For these experiments, we used two 30 bp DNA probes, B12 and B34, which share the same DNA sequences but possess PT-modified 5’-GPSAAC-3’/5’-GPSTTC-3’ and non-PT-modified 5’-GAAC-3’/5’-GTTC-3’ motifs, respectively.
HostingRepository	PRIDE
AnnounceDate	2022-02-26
AnnouncementXML	Submission_2022-02-26_07:46:22.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	You Tang
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Cerro; NCBI TaxID: 340188;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 10:01:54	ID requested
⏵ 1	2022-02-26 07:46:22	announced

Dataset with its publication pending

submitter keyword: phosphorothioation modification, Salmonella，Q Exactive

You Tang
contact affiliation	Wuhan University
contact email	tangyou.cool@163.com
lab head
You Tang
contact affiliation	Wuhan University
contact email	tangyou.cool@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031869
     1. Label: PRIDE project
     2. Name: PT-modified B12 probe and Non-PT-modified B34 probe pull-down with Salmonella enterica serovar Cerro 87 cell lysate