PXD031861

PXD031861 is an original dataset announced via ProteomeXchange.

Title	Trypanosoma evansi secretome carries potential biomarkers for surra diagnosis
Description	Trypanosoma evansi is a parasite of African origin that has evolutionary proximity to Trypanosoma brucei. This parasite, which causes the surra, has the broadest range of hosts, including wild and domestic mammals, and its geographic distribution is the widest among all trypanosomes. This disease causes numerous economic losses in livestock in countries in the Americas, Africa, and Asia. This work aims to obtain in vitro secreted proteins from T. evansi and identify potential treatment and diagnostic biomarkers of surra. For this, in an experimental group, the parasite was purified in an ion-exchange column (DEAE-Cellulose) and maintained in a secretion medium. In a second group, there was no purification of the parasites. Also, each group was maintained at 37 ºC and 27 ºC. Finally, we identified the proteins through mass spectrometry. We found a total of 246 proteins and noticed that the temperature appears to modulate protein secretion. We observed negligible differences in proteins from purified and non-purified sets. We observed that most secreted proteins are related to molecular function (F) of binding, catalytic activity, structural molecule activity, and biological process (P) of metabolic process, cellular process, localization, and biological regulation. We also verified that most secretome proteins have homologous proteins with trypanosomatids T. brucei, T. b. gambiense, T. vivax, and T. equiperdum. However, despite this, it was possible to identify the presence of exclusive T. evansi epitopes with potential biomarkers for differential surra diagnosis. We identified epitopes of proteins that are part of the glycolytic process of the parasite, which can be used in immunotherapy by the production of anti-proteins from the glycolytic process. Finally, this is the first work that provide a big picture of the T. evansi secretome and could be the starting point to elucidate biological issues related to surra.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:15.680.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031861
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Renato Moreira
SpeciesList	scientific name: Trypanosoma evansi; NCBI TaxID: 5697;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 00:57:33	ID requested
1	2023-03-04 11:16:38	announced
⏵ 2	2023-11-14 08:28:16	announced	2023-11-14: Updated project metadata.

10.6019/PXD031861;

Moreira RS, Calomeno NA, das Neves GB, do Nascimento LFN, Filho VB, Wagner G, Miletti LC, Trypanosoma evansi secretome carries potential biomarkers for Surra diagnosis. J Proteomics, 272():104789(2023) [pubmed]

submitter keyword: Proteomic

Bioinformatic

Immunoinformatic

Epitope;

Renato Simões Moreira
contact affiliation	Professor at Federal Institute of Santa Catarina
contact email	renatosm@gmail.com
lab head
Renato Moreira
contact affiliation	Federal Institute of Santa Catarina
contact email	renatosm@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031861

PRIDE project URI

• PRIDE
  1. PXD031861
     1. Label: PRIDE project
     2. Name: Trypanosoma evansi secretome carries potential biomarkers for surra diagnosis