PXD031852
PXD031852 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration |
Description | Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and inflammation. Conventional strategies, such as the control of implant microstructure or the introduction of growth factors, have limited functions failing to manage these complex environments. Here we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes (CuTA@SF) to provide a suitable microenvironment for enhanced OCD regeneration. The incorporation of CuTA nanozymes endowed the SF hydrogel with a uniform microstructure and elevated hydrophilicity. In vitro cultivation of mesenchymal stem cells (MSCs) and chondrocytes showed that CuTA@SF hydrogel accelerated cell proliferation and enhanced cell viability, as well as had antioxidant and antibacterial properties. Under the inflammatory environment with the stimulation of IL-1β, CuTA@SF hydrogel still possessed the potential to promote MSC osteogenesis and deposition of cartilage-specific extracellular matrix (ECM). The proteomics analysis further confirmed that CuTA@SF hydrogel promoted cell proliferation and ECM synthesis. In the full-thickness OCD model of rabbit, CuTA@SF hydrogel displayed successfully in situ OCD regeneration, as evidenced by micro-CT, histology (HE, S/O, and toluidine blue staining) and immunohistochemistry (Col I and aggrecan immunostaining). Therefore, CuTA@SF hydrogel is a promising biomaterial targeted at the regeneration of OCD. |
HostingRepository | iProX |
AnnounceDate | 2022-03-04 |
AnnouncementXML | Submission_2022-10-08_00:36:30.724.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhicheng Cao |
SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-02-23 17:53:13 | ID requested | |
1 | 2022-02-23 17:53:41 | announced | |
⏵ 2 | 2022-10-08 00:36:31 | announced | 2022-10-08: Update publication information. |
Publication List
Cao Z, Wang H, Chen J, Zhang Y, Mo Q, Zhang P, Wang M, Liu H, Bao X, Sun Y, Zhang W, Yao Q, Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration. Bioact Mater, 20():221-242(2023) [pubmed] |
Keyword List
submitter keyword: osteochondral regeneration, metal-organic framework, nanozyme, inflammation, ROS, silk |
Contact List
Wei Zhang | |
---|---|
contact affiliation | Southeast University |
contact email | zhang.wei@seu.edu.cn |
lab head | |
Zhicheng Cao | |
contact affiliation | Nanjing Medical University |
contact email | caozhichengfxy@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |