PXD031852

PXD031852 is an original dataset announced via ProteomeXchange.

Title	Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration
Description	Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and inflammation. Conventional strategies, such as the control of implant microstructure or the introduction of growth factors, have limited functions failing to manage these complex environments. Here we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes (CuTA@SF) to provide a suitable microenvironment for enhanced OCD regeneration. The incorporation of CuTA nanozymes endowed the SF hydrogel with a uniform microstructure and elevated hydrophilicity. In vitro cultivation of mesenchymal stem cells (MSCs) and chondrocytes showed that CuTA@SF hydrogel accelerated cell proliferation and enhanced cell viability, as well as had antioxidant and antibacterial properties. Under the inflammatory environment with the stimulation of IL-1β, CuTA@SF hydrogel still possessed the potential to promote MSC osteogenesis and deposition of cartilage-specific extracellular matrix (ECM). The proteomics analysis further confirmed that CuTA@SF hydrogel promoted cell proliferation and ECM synthesis. In the full-thickness OCD model of rabbit, CuTA@SF hydrogel displayed successfully in situ OCD regeneration, as evidenced by micro-CT, histology (HE, S/O, and toluidine blue staining) and immunohistochemistry (Col I and aggrecan immunostaining). Therefore, CuTA@SF hydrogel is a promising biomaterial targeted at the regeneration of OCD.
HostingRepository	iProX
AnnounceDate	2022-03-04
AnnouncementXML	Submission_2022-10-08_00:36:30.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhicheng Cao
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-23 17:53:13	ID requested
1	2022-02-23 17:53:41	announced
⏵ 2	2022-10-08 00:36:31	announced	2022-10-08: Update publication information.

Cao Z, Wang H, Chen J, Zhang Y, Mo Q, Zhang P, Wang M, Liu H, Bao X, Sun Y, Zhang W, Yao Q, Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration. Bioact Mater, 20():221-242(2023) [pubmed]

submitter keyword: osteochondral regeneration, metal-organic framework, nanozyme, inflammation, ROS, silk

Wei Zhang
contact affiliation	Southeast University
contact email	zhang.wei@seu.edu.cn
lab head
Zhicheng Cao
contact affiliation	Nanjing Medical University
contact email	caozhichengfxy@163.com
dataset submitter

iProX dataset URI